Chitosan/alginate complexes were prepared at different polycation/polyanion molar ratios and freezedried vaginal inserts were obtained for chlorhexidine digluconate local delivery in genital infections. Complex yield, FT-IR spectra, and TGA thermograms were studied to confirm the interaction between the two polyions. The influence of different complexes on physical handling, morphology, and drug distribution in the samples were evaluated by friability test, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS), respectively. In vitro water-uptake, mucoadhesion and release tests were performed as well as microbiological tests toward pathogenic vaginal microorganisms. The results showed that the selection of suitable chitosan/alginate molar ratio and drug loading allowed modulate insert ability to hydrate, adhere to the mucosa, and release chlorhexidine digluconate. The insert containing an excess of alginate was found to be the best performing formulation and showed good antimicrobial activity toward the pathogens Candida albicans and Escherichia coli.
A. Abruzzo, F. Bigucci, T. Cerchiara, B. Saladini, M.C. Gallucci, F. Cruciani, et al. (2013). Chitosan/alginate complexes for vaginal delivery of chlorhexidine digluconate. CARBOHYDRATE POLYMERS, 91, 651-658 [10.1016/j.carbpol.2012.08.074].
Chitosan/alginate complexes for vaginal delivery of chlorhexidine digluconate
ABRUZZO, ANGELA;BIGUCCI, FEDERICA;CERCHIARA, TERESA;SALADINI, BRUNO;CRUCIANI, FEDERICA;VITALI, BEATRICE;LUPPI, BARBARA
2013
Abstract
Chitosan/alginate complexes were prepared at different polycation/polyanion molar ratios and freezedried vaginal inserts were obtained for chlorhexidine digluconate local delivery in genital infections. Complex yield, FT-IR spectra, and TGA thermograms were studied to confirm the interaction between the two polyions. The influence of different complexes on physical handling, morphology, and drug distribution in the samples were evaluated by friability test, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS), respectively. In vitro water-uptake, mucoadhesion and release tests were performed as well as microbiological tests toward pathogenic vaginal microorganisms. The results showed that the selection of suitable chitosan/alginate molar ratio and drug loading allowed modulate insert ability to hydrate, adhere to the mucosa, and release chlorhexidine digluconate. The insert containing an excess of alginate was found to be the best performing formulation and showed good antimicrobial activity toward the pathogens Candida albicans and Escherichia coli.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
