Background. Portal vein arterialization (PVA) has shown efficacy to treat acute liver failure (ALF) in preclinical studies. The next step is to perform large animal studies that propose a clinically acceptable method of PVA. In this study, we assessed the efficacy of PVA using an extracorporeal device to treat 2 ALF models in swine. Materials and Methods. The 2 ALF swine models were carbon tetrachloride toxic ALF and subtotal hepatectomy using 8 animals per group. PVA was performed with an extracorporeal device that may be suitable for future clinical studies. Arterial blood was drawn from the iliac artery and delivered into the portal vein for a 6-hour treatment. We analyzed biochemical, blood gas, and histological parameters as well as 1-week survival rates. Results. In both models, ALF was successfully achieved. Control group animals deteriorated biochemically, dropping their prothrombin times and increasing the liver enzymes. In contrast, treated animals improved with a survival rate of 75% at 7 days compared with 0% for the former group. Conclusions. PVA using an extracorporeal device was feasible and effective to treat both toxic and resective ALF in swine.

Nardo B, Tsivian M, Neri F, Piras G, Pariali M, Bertelli R, et al. (2008). Extracorporeal portal vein oxygenation improves outcome of acute liver failure in swine. TRANSPLANTATION PROCEEDINGS, 40, 2046-2048 [10.1016/j.transproceed.2008.05.026].

Extracorporeal portal vein oxygenation improves outcome of acute liver failure in swine

NARDO, BRUNO;NERI, FLAVIA;BERTELLI, RICCARDO;CAVALLARI, GIUSEPPE
2008

Abstract

Background. Portal vein arterialization (PVA) has shown efficacy to treat acute liver failure (ALF) in preclinical studies. The next step is to perform large animal studies that propose a clinically acceptable method of PVA. In this study, we assessed the efficacy of PVA using an extracorporeal device to treat 2 ALF models in swine. Materials and Methods. The 2 ALF swine models were carbon tetrachloride toxic ALF and subtotal hepatectomy using 8 animals per group. PVA was performed with an extracorporeal device that may be suitable for future clinical studies. Arterial blood was drawn from the iliac artery and delivered into the portal vein for a 6-hour treatment. We analyzed biochemical, blood gas, and histological parameters as well as 1-week survival rates. Results. In both models, ALF was successfully achieved. Control group animals deteriorated biochemically, dropping their prothrombin times and increasing the liver enzymes. In contrast, treated animals improved with a survival rate of 75% at 7 days compared with 0% for the former group. Conclusions. PVA using an extracorporeal device was feasible and effective to treat both toxic and resective ALF in swine.
2008
Nardo B, Tsivian M, Neri F, Piras G, Pariali M, Bertelli R, et al. (2008). Extracorporeal portal vein oxygenation improves outcome of acute liver failure in swine. TRANSPLANTATION PROCEEDINGS, 40, 2046-2048 [10.1016/j.transproceed.2008.05.026].
Nardo B; Tsivian M; Neri F; Piras G; Pariali M; Bertelli R; Cavallari G
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/126443
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 9
social impact