A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiation
Titolo: | A system biology approach to ionizing radiation response by mammalian cell lines | |
Autore/i: | VIRELLI, ANGELA; GAVOCI, ENTELE; CASTELLANI, GASTONE; ZIRONI, ISABELLA; CECCOLINI, ELISA; ROCCHI, FEDERICO; SUMINI, MARCO; E. Belligotti | |
Autore/i Unibo: | ||
Anno: | 2011 | |
Titolo del libro: | Proceedings of the SysBioHealth Symposium 2011 | |
Pagina iniziale: | 99 | |
Pagina finale: | 104 | |
Abstract: | A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiation | |
Data prodotto definitivo in UGOV: | 28-giu-2013 | |
Appare nelle tipologie: | 4.01 Contributo in Atti di convegno |