A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiation
A. Virelli, E. Gavoci, G. Castellani, I. Zironi, E. Ceccolini, F. Rocchi, et al. (2011). A system biology approach to ionizing radiation response by mammalian cell lines. BOLOGNA : Bononia University Press.
A system biology approach to ionizing radiation response by mammalian cell lines
VIRELLI, ANGELA;GAVOCI, ENTELE;CASTELLANI, GASTONE;ZIRONI, ISABELLA;CECCOLINI, ELISA;ROCCHI, FEDERICO;SUMINI, MARCO;
2011
Abstract
A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiationI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
