The usefulness of RFLP analyses on a 435 bp fragment of the tuf gene for preliminary identification of phytoplasmas from a number of phytoplasma ribosomal groups and/or ‘Candidatus Phytoplasma’ was verified. The strains employed belong to 13 16Sr DNA groups and to 22 different subgroups and were both from periwinkle infected plants and from naturally infected plants. The combined RFLP patterns obtained with the three restriction enzymes employed allow the distinction of a total of 18 differ-ent profiles, however no discrimination is provided for some of the ribosomal group for which sequencing remains the main tool for phytoplasma identification.
Use of a fragment of the tuf gene for phytoplasma 16Sr group/subgroup differentiation / Contaldo N.; A. Canel; O. Makarova; S. Paltrinieri; A. Bertaccini; M. Nicolaisen. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 64 supplement:(2011), pp. S45-S46.
Use of a fragment of the tuf gene for phytoplasma 16Sr group/subgroup differentiation
CONTALDO, NICOLETTA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA;
2011
Abstract
The usefulness of RFLP analyses on a 435 bp fragment of the tuf gene for preliminary identification of phytoplasmas from a number of phytoplasma ribosomal groups and/or ‘Candidatus Phytoplasma’ was verified. The strains employed belong to 13 16Sr DNA groups and to 22 different subgroups and were both from periwinkle infected plants and from naturally infected plants. The combined RFLP patterns obtained with the three restriction enzymes employed allow the distinction of a total of 18 differ-ent profiles, however no discrimination is provided for some of the ribosomal group for which sequencing remains the main tool for phytoplasma identification.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
