Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of ‘Candidatus Phytoplasma asteris’ in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation in strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.
Mitrović J., Contaldo N., Paltrinieri S., Meja J.F., Mori N., Bertaccini A., et al. (2011). The use of groEL gene in characterisation of aster yellows phytoplasmas in field collected samples. BULLETIN OF INSECTOLOGY, 64 supplement, S17-S18.
The use of groEL gene in characterisation of aster yellows phytoplasmas in field collected samples
CONTALDO, NICOLETTA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA;DUDUK, BOJAN
2011
Abstract
Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of ‘Candidatus Phytoplasma asteris’ in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation in strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
