Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of ‘Candidatus Phytoplasma asteris’ in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation in strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.
The use of groEL gene in characterisation of aster yellows phytoplasmas in field collected samples / Mitrović J.; Contaldo N.; Paltrinieri S.; Meja J.F.; Mori N.; Bertaccini A.; Duduk B. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 64 supplement:(2011), pp. S17-S18.
The use of groEL gene in characterisation of aster yellows phytoplasmas in field collected samples
CONTALDO, NICOLETTA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA;DUDUK, BOJAN
2011
Abstract
Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of ‘Candidatus Phytoplasma asteris’ in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation in strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
