The diversity of phytoplasmas within single plants has not yet been fully investigated. In this project, deep amplicon sequencing was used to generate 50,926 phytoplasma sequences from 11 phytoplasma-infected samples from a PCR amplicon in the 5’ end of the 16S region. After clustering and alignment to reference sequences it was shown that the phytoplasmas in the 11 plants be-longed to several 16Sr groups and that a high number of single nucleotide polymorphisms were present.
Deep amplicon sequencing reveals mixed phytoplasma infection within single grapevine plants / Nicolaisen M.; N. Contaldo; O. Makarova; S. Paltrinieri; A. Bertaccini. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 64 supplement:(2011), pp. S35-S36.
Deep amplicon sequencing reveals mixed phytoplasma infection within single grapevine plants
CONTALDO, NICOLETTA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2011
Abstract
The diversity of phytoplasmas within single plants has not yet been fully investigated. In this project, deep amplicon sequencing was used to generate 50,926 phytoplasma sequences from 11 phytoplasma-infected samples from a PCR amplicon in the 5’ end of the 16S region. After clustering and alignment to reference sequences it was shown that the phytoplasmas in the 11 plants be-longed to several 16Sr groups and that a high number of single nucleotide polymorphisms were present.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
