During summer 2006 sweet cherry (Prunus avium L) trees, exhibiting disease symptoms suggestive of possible phytoplasma in-fection were observed in the Libertador General Bernardo O’Higgins region of Chile. Branches were collected from 10 plants (5 with and 5 without symptoms) and nucleic acid extracted from phloem scrapings were used for phytoplasmas testing. Nested po-lymerase chain reaction (PCR) amplification allows the detection of phytoplasmas in samples from plants that showed symptoms, but not in those from asymptomatic ones. Restriction fragment length polymorphism, cloning, and sequencing allowed identifica-tion of phytoplasmas into ribosomal subgroup 16SrIII-J.
Identification of phytoplasma belonging to X-disease group in cherry in Chile / González F.; A. Zamorano; A.M. Pino; S. Paltrinieri; A. Bertaccini; N. Fiore. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 64 supplement:(2011), pp. S235-S236.
Identification of phytoplasma belonging to X-disease group in cherry in Chile
PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA;
2011
Abstract
During summer 2006 sweet cherry (Prunus avium L) trees, exhibiting disease symptoms suggestive of possible phytoplasma in-fection were observed in the Libertador General Bernardo O’Higgins region of Chile. Branches were collected from 10 plants (5 with and 5 without symptoms) and nucleic acid extracted from phloem scrapings were used for phytoplasmas testing. Nested po-lymerase chain reaction (PCR) amplification allows the detection of phytoplasmas in samples from plants that showed symptoms, but not in those from asymptomatic ones. Restriction fragment length polymorphism, cloning, and sequencing allowed identifica-tion of phytoplasmas into ribosomal subgroup 16SrIII-J.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
