A total of 154 phytoplasma strains from 15 ribosomal groups were employed for barcode sequences production. Besides strains in periwinkle, 36 strains in natural infected plants such as napier grass, grapevine, plum, jujube, apple, pear, spartium, pine tree, hibiscus and erigeron were employed. Barcode sequences were produced for 16SrDNA, tuf and SecA gene for 36 phytoplasma strains, while for 54 strains 16SrDNA sequences were obtained, and for 118 and 89 strains respectively the tuf and secA barcode. All obtained sequences and protocol for extraction and PCR amplification will be available in Qbank.
Bertaccini A., S. Paltrinieri, O. Makarova, N. Contaldo, M. Nicolaisen (2011). Sharing information and collections on phytoplasmas: from QBOL to QBANK. BULLETIN OF INSECTOLOGY, 64 supplement, S289-S290.
Sharing information and collections on phytoplasmas: from QBOL to QBANK
BERTACCINI, ASSUNTA;PALTRINIERI, SAMANTA;CONTALDO, NICOLETTA;
2011
Abstract
A total of 154 phytoplasma strains from 15 ribosomal groups were employed for barcode sequences production. Besides strains in periwinkle, 36 strains in natural infected plants such as napier grass, grapevine, plum, jujube, apple, pear, spartium, pine tree, hibiscus and erigeron were employed. Barcode sequences were produced for 16SrDNA, tuf and SecA gene for 36 phytoplasma strains, while for 54 strains 16SrDNA sequences were obtained, and for 118 and 89 strains respectively the tuf and secA barcode. All obtained sequences and protocol for extraction and PCR amplification will be available in Qbank.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
