In the present study, a monoclonal antibody, D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. negevensis infected cells, by using the immunofluorescence test. In order to identify by immunoblotting the protein recognized by D5-14 mAb, S. negevensis and C. trachomatis LGV2 elementary bodies (Ebs) were purified by sucrose-density-gradient. The D5-14 mAb, reacting in immublot with a 66 kDa protein of C. trachomatis. recognized a protein with the same moleculr mass of 66 kda when tested with S. negevensis.
DONATI M., DI PAOLO M., AVANZI S., DI FRANCESCO A., FIANI N., CEVENINI R. (2012). Detection of Simkania negevensis in cell culture by using a monoclonal antibody. AMSTERDAM : Sander Ousburg & Servaas A. Morré.
Detection of Simkania negevensis in cell culture by using a monoclonal antibody
DONATI, MANUELA;DI FRANCESCO, ANTONIETTA;CEVENINI, ROBERTO
2012
Abstract
In the present study, a monoclonal antibody, D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. negevensis infected cells, by using the immunofluorescence test. In order to identify by immunoblotting the protein recognized by D5-14 mAb, S. negevensis and C. trachomatis LGV2 elementary bodies (Ebs) were purified by sucrose-density-gradient. The D5-14 mAb, reacting in immublot with a 66 kDa protein of C. trachomatis. recognized a protein with the same moleculr mass of 66 kda when tested with S. negevensis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
