In this paper, we describe a new method to obtain D2O/D2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.
Malferrari M., Venturoli G., Francia F., Mezzetti A. (2012). A New Method for D2O/H2O Exchange in Infrared Spectroscopy of Proteins. SPECTROSCOPY, 27, 337-342 [10.1155/2012/791342].
A New Method for D2O/H2O Exchange in Infrared Spectroscopy of Proteins
MALFERRARI, MARCO;VENTUROLI, GIOVANNI;FRANCIA, FRANCESCO;
2012
Abstract
In this paper, we describe a new method to obtain D2O/D2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
