In this paper, we describe a new method to obtain D2O/D2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.
A New Method for D2O/H2O Exchange in Infrared Spectroscopy of Proteins / Malferrari M.; Venturoli G.; Francia F.; Mezzetti A.. - In: SPECTROSCOPY. - ISSN 0712-4813. - STAMPA. - 27:(2012), pp. 337-342. [10.1155/2012/791342]
A New Method for D2O/H2O Exchange in Infrared Spectroscopy of Proteins
MALFERRARI, MARCO;VENTUROLI, GIOVANNI;FRANCIA, FRANCESCO;
2012
Abstract
In this paper, we describe a new method to obtain D2O/D2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.