Bloodstream infections are associated with high morbidity and mortality. Blood culture is considered to be the most important tool in their diagnosis. In this study we compared standard blood culture with two different molecular techniques (Prove-it Sepsis and LightCycler SeptiFast M) to detect bacterial species and fungi in the blood of septic patients. Both methods showed significant agreement with the results obtained by blood culture. The results of these studies suggest that both the LightCycler SeptiFast and Prove-it Sepsis tests can be used for the rapid and specific diagnosis of bacteraemia and bloodstream infections. It is important, however, to emphasize that molecular methods cannot replace blood cultures, as the latter allow the evaluation of the antimicrobial susceptibility of isolated microbes
Gaibani P, Rossini G, Ambretti S, Gelsomino F, Pierro AM, Varani S, et al. (2009). Blood culture systems: rapid detection-how and why?. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 34(s4), 13-15 [10.1016/S0924-8579(09)70559-X].
Blood culture systems: rapid detection-how and why?
GAIBANI, PAOLO;VARANI, STEFANIA;LANDINI, MARIA PAOLA;SAMBRI, VITTORIO
2009
Abstract
Bloodstream infections are associated with high morbidity and mortality. Blood culture is considered to be the most important tool in their diagnosis. In this study we compared standard blood culture with two different molecular techniques (Prove-it Sepsis and LightCycler SeptiFast M) to detect bacterial species and fungi in the blood of septic patients. Both methods showed significant agreement with the results obtained by blood culture. The results of these studies suggest that both the LightCycler SeptiFast and Prove-it Sepsis tests can be used for the rapid and specific diagnosis of bacteraemia and bloodstream infections. It is important, however, to emphasize that molecular methods cannot replace blood cultures, as the latter allow the evaluation of the antimicrobial susceptibility of isolated microbesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.