Purpose: Bronchiolitis obliterans is the major factor limiting long-term success of lung transplantation. Gene polymorphisms of surfactant proteins, key players in lung innate immunity, have been associated with various lung diseases. The aim of this study was to investigate the association between donor lung SP-D gene polymorphisms with recipient post transplant bronchiolitis obliterans syndrome and survival. Methods and Materials: Lung-Tx pts (192) were prospectively followed by PFTs, and bronchoscopies with BAL and biopsies. Donor DNA was assayed for SP-D gene polymorphisms of two single-nucleotide variations altering amino acids in the mature protein in codon 11 (Met11Thr) of the N-terminal domain, and in codon 160 (Ala160Thr) of the C-terminal domain, using the pyrosequencing method. Results: BOS was diagnosed in 49 pts. The table shows the SP-D genotype frequency for codon 11 (Met11Thr), and codon 160 (Ala160Thr). Recipients of lung allografts with the Thr/Thr11-encoding genotype had significantly earlier BOS development and reduced survival compared to those with the Met/Met11-encoding genotypes. No significant association was noted for SP-D variants Ala/Thr160.
Aramini B., Diangelo S., Lederer D., Wilt J., Shah L., Mattioli S., et al. (2011). Donor Lung Surfactant Protein D Gene Polymorphisms Are Associated with Bronchiolitis Obliterans Syndrome and Mortality after Lung Transplantation. THE JOURNAL OF HEART AND LUNG TRANSPLANTATION, 30; 4 (S)(4 (S)), S56-S56.
Donor Lung Surfactant Protein D Gene Polymorphisms Are Associated with Bronchiolitis Obliterans Syndrome and Mortality after Lung Transplantation
ARAMINI, BEATRICE;MATTIOLI, SANDRO;
2011
Abstract
Purpose: Bronchiolitis obliterans is the major factor limiting long-term success of lung transplantation. Gene polymorphisms of surfactant proteins, key players in lung innate immunity, have been associated with various lung diseases. The aim of this study was to investigate the association between donor lung SP-D gene polymorphisms with recipient post transplant bronchiolitis obliterans syndrome and survival. Methods and Materials: Lung-Tx pts (192) were prospectively followed by PFTs, and bronchoscopies with BAL and biopsies. Donor DNA was assayed for SP-D gene polymorphisms of two single-nucleotide variations altering amino acids in the mature protein in codon 11 (Met11Thr) of the N-terminal domain, and in codon 160 (Ala160Thr) of the C-terminal domain, using the pyrosequencing method. Results: BOS was diagnosed in 49 pts. The table shows the SP-D genotype frequency for codon 11 (Met11Thr), and codon 160 (Ala160Thr). Recipients of lung allografts with the Thr/Thr11-encoding genotype had significantly earlier BOS development and reduced survival compared to those with the Met/Met11-encoding genotypes. No significant association was noted for SP-D variants Ala/Thr160.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
