The caudices of ten Adenia species contain galactose-binding lectins that were purified by affinity chromatography. All lectins but three agglutinate human erythrocytes. Six lectins consist of two unequal chains, which can be separated by reduction, and inhibit protein synthesis both by a rabbit reticulocyte lysate and by HeLa and Raji cells. The lectins from A. goetzii, A. lanceolata and A. stenodactyla had the highest cytotoxicity, inhibiting cell protein synthesis with IC50’s (concentration inhibiting by 50%) below 0.1 ng/ml, and deadenylate DNA, thus being type 2 ribosome-inactivating proteins.

PELOSI E., LUBELLI C., POLITO L., BARBIERI L., BOLOGNESI A., STIRPE F. (2005). Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae). TOXICON, 46, 658-663 [10.1016/j.toxicon.2005.07.008].

Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae)

PELOSI, EMANUELE;LUBELLI, CHIARA;POLITO, LETIZIA;BARBIERI, LUIGI;BOLOGNESI, ANDREA;STIRPE, FIORENZO
2005

Abstract

The caudices of ten Adenia species contain galactose-binding lectins that were purified by affinity chromatography. All lectins but three agglutinate human erythrocytes. Six lectins consist of two unequal chains, which can be separated by reduction, and inhibit protein synthesis both by a rabbit reticulocyte lysate and by HeLa and Raji cells. The lectins from A. goetzii, A. lanceolata and A. stenodactyla had the highest cytotoxicity, inhibiting cell protein synthesis with IC50’s (concentration inhibiting by 50%) below 0.1 ng/ml, and deadenylate DNA, thus being type 2 ribosome-inactivating proteins.
2005
PELOSI E., LUBELLI C., POLITO L., BARBIERI L., BOLOGNESI A., STIRPE F. (2005). Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae). TOXICON, 46, 658-663 [10.1016/j.toxicon.2005.07.008].
PELOSI E.; LUBELLI C.; POLITO L.; BARBIERI L.; BOLOGNESI A.; STIRPE F.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/11816
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