The spray congealing technique, a solvent-free drug encapsulation process, was succesfuly employed to obtain lipid-based particulate systems with high (10-20% w/w) protein loading. Bovine serum albumine (BSA) was utilised as model protein and three low melting lipids (glyceryl palmitostearate, trimirystin and tristearin) were employed as carriers. BSA-loaded lipid microparticles were characterized in terms of particle size, morphology and drug loading. The results showed that the microparticles exhibited a spherical shape, mean diameter in the range 150-300 mm and an encapsulation efficiency higher than 90%. Possible changes in the protein structure as a result of the manufacturing process was then investigated for the first time using UV spectrophotometry in fourth derivative mode and FT-Raman spectroscopy. The results suggested that the structural integrity of the protein was maintained within the particles. Thermal analysis indicated that the effect of protein on the thermal properties of the carriers could be detected. Spray congealing could thus be considered a suitable technique to produce highly BSA loaded microparticles preserving the structure of the protein.

SPRAY CONGEALED LIPID MICROPARTICLES WITH HIGH PROTEIN LOADING: PREPARATION AND SOLID STATE CHARACTERIZATION / M. Di Sabatino; B. Albertini; V.L. Kett; N. Passerini. - In: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES. - ISSN 0928-0987. - STAMPA. - 46:(2012), pp. 346-356. [10.1016/j.ejps.2012.02.021]

SPRAY CONGEALED LIPID MICROPARTICLES WITH HIGH PROTEIN LOADING: PREPARATION AND SOLID STATE CHARACTERIZATION

DI SABATINO, MARCELLO;ALBERTINI, BEATRICE;PASSERINI, NADIA
2012

Abstract

The spray congealing technique, a solvent-free drug encapsulation process, was succesfuly employed to obtain lipid-based particulate systems with high (10-20% w/w) protein loading. Bovine serum albumine (BSA) was utilised as model protein and three low melting lipids (glyceryl palmitostearate, trimirystin and tristearin) were employed as carriers. BSA-loaded lipid microparticles were characterized in terms of particle size, morphology and drug loading. The results showed that the microparticles exhibited a spherical shape, mean diameter in the range 150-300 mm and an encapsulation efficiency higher than 90%. Possible changes in the protein structure as a result of the manufacturing process was then investigated for the first time using UV spectrophotometry in fourth derivative mode and FT-Raman spectroscopy. The results suggested that the structural integrity of the protein was maintained within the particles. Thermal analysis indicated that the effect of protein on the thermal properties of the carriers could be detected. Spray congealing could thus be considered a suitable technique to produce highly BSA loaded microparticles preserving the structure of the protein.
2012
SPRAY CONGEALED LIPID MICROPARTICLES WITH HIGH PROTEIN LOADING: PREPARATION AND SOLID STATE CHARACTERIZATION / M. Di Sabatino; B. Albertini; V.L. Kett; N. Passerini. - In: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES. - ISSN 0928-0987. - STAMPA. - 46:(2012), pp. 346-356. [10.1016/j.ejps.2012.02.021]
M. Di Sabatino; B. Albertini; V.L. Kett; N. Passerini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/118037
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