Nicotine dependence is known to induce long-term neural adaptations in brain. The purpose of this study was to verify whether specific protein patterns related to nicotine self administration states could also be detected in a peripheral tissue. A serum proteomic analysis was performed by 2-DE on samples taken at six time points: N, naïve; P, priming; S, self-administration; W, withdrawal; E, extinction; R, relapse. After image analysis, spot volume values were submitted to a principal component analysis and relevant comparisons were selected. In N versus S; S versus W; E versus R; S versus R and S versus E comparisons a clear separation between groups could be observed, suggesting that each self-administration state correlates with a specific protein expression pattern. Partial least squares discriminant analysis was adopted to rank proteins by the contribution to the overall separation. A number of spots were identified; among them, C reactive protein and haemopexin displayed a significant reduction after nicotine administration; two haemopexin isoforms were decreased in the S state and antithrombin III was increased in the E phase. This study showed that specific protein patterns related to the nicotine self-administration states exist in serum. Further development of this approach may provide biomarkers to assess dependence states of drug-taking individuals.

Cecconi D., Tessari M., Willé D.R., Zoli M., Domenici E., Righetti P.G., et al. (2008). Serum proteomic analysis during nicotine self-administration, extinction and relapse in rats. ELECTROPHORESIS, 29, 1525-1533 [10.1002/elps.200700388].

Serum proteomic analysis during nicotine self-administration, extinction and relapse in rats.

CARBONI, LUCIA
2008

Abstract

Nicotine dependence is known to induce long-term neural adaptations in brain. The purpose of this study was to verify whether specific protein patterns related to nicotine self administration states could also be detected in a peripheral tissue. A serum proteomic analysis was performed by 2-DE on samples taken at six time points: N, naïve; P, priming; S, self-administration; W, withdrawal; E, extinction; R, relapse. After image analysis, spot volume values were submitted to a principal component analysis and relevant comparisons were selected. In N versus S; S versus W; E versus R; S versus R and S versus E comparisons a clear separation between groups could be observed, suggesting that each self-administration state correlates with a specific protein expression pattern. Partial least squares discriminant analysis was adopted to rank proteins by the contribution to the overall separation. A number of spots were identified; among them, C reactive protein and haemopexin displayed a significant reduction after nicotine administration; two haemopexin isoforms were decreased in the S state and antithrombin III was increased in the E phase. This study showed that specific protein patterns related to the nicotine self-administration states exist in serum. Further development of this approach may provide biomarkers to assess dependence states of drug-taking individuals.
2008
Cecconi D., Tessari M., Willé D.R., Zoli M., Domenici E., Righetti P.G., et al. (2008). Serum proteomic analysis during nicotine self-administration, extinction and relapse in rats. ELECTROPHORESIS, 29, 1525-1533 [10.1002/elps.200700388].
Cecconi D.; Tessari M.; Willé D.R.; Zoli M.; Domenici E.; Righetti P.G.; Carboni L.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/117562
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