A diagnostic device suitable for Parvovirus B19 DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications. The device was employed to analyze 50 serum clinical samples, upon PCR amplification.
Parvovirus B19 DNA detection in serum samples employing a microfluidic device based on chemiluminescence contact imaging.
MIRASOLI, MARA;DOLCI, LUISA STELLA;BONVICINI, FRANCESCA;BURAGINA, ANGELA;DI FURIO, FRANCESCA;ZANGHERI, MARTINA;GUARDIGLI, MASSIMO;GALLINELLA, GIORGIO;RODA, ALDO
2012
Abstract
A diagnostic device suitable for Parvovirus B19 DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications. The device was employed to analyze 50 serum clinical samples, upon PCR amplification.File in questo prodotto:
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