A diagnostic device suitable for Parvovirus B19 DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications. The device was employed to analyze 50 serum clinical samples, upon PCR amplification.

Parvovirus B19 DNA detection in serum samples employing a microfluidic device based on chemiluminescence contact imaging.

MIRASOLI, MARA;DOLCI, LUISA STELLA;BONVICINI, FRANCESCA;BURAGINA, ANGELA;DI FURIO, FRANCESCA;ZANGHERI, MARTINA;GUARDIGLI, MASSIMO;GALLINELLA, GIORGIO;RODA, ALDO
2012

Abstract

A diagnostic device suitable for Parvovirus B19 DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications. The device was employed to analyze 50 serum clinical samples, upon PCR amplification.
M.Mirasoli; L.S.Dolci; F.Bonvicini; A.Buragina; F.Di Furio; M.Zangheri; M.Guardigli; G.Gallinella; A.Roda
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/117477
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