A diagnostic device suitable for Parvovirus B19 DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications. The device was employed to analyze 50 serum clinical samples, upon PCR amplification.
M.Mirasoli, L.S.Dolci, F.Bonvicini, A.Buragina, F.Di Furio, M.Zangheri, et al. (2012). Parvovirus B19 DNA detection in serum samples employing a microfluidic device based on chemiluminescence contact imaging. LUMINESCENCE, 27, 141-142.
Parvovirus B19 DNA detection in serum samples employing a microfluidic device based on chemiluminescence contact imaging.
MIRASOLI, MARA;DOLCI, LUISA STELLA;BONVICINI, FRANCESCA;BURAGINA, ANGELA;DI FURIO, FRANCESCA;ZANGHERI, MARTINA;GUARDIGLI, MASSIMO;GALLINELLA, GIORGIO;RODA, ALDO
2012
Abstract
A diagnostic device suitable for Parvovirus B19 DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications. The device was employed to analyze 50 serum clinical samples, upon PCR amplification.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
