The majority of methods, with exception of electron paramagnetic resonance, used to evaluate the identity, the rate and the conditions of the reactive oxygen species produced by mitochondria, are mainly based on oxidation sensitive markers. Following latest electrochemical methodology, we implemented a novel electrochemical assay for the investigation of aerobic metabolism in preparations of isolated mitochondria through simultaneous measurement of O-2 consumption and reactive species production. This electrochemical assay reveals active H2O2 production by respiring mouse liver mitochondria, and shows that ATP synthase activation and moderate depolarization increase the rate of H2O2 formation, suggesting that ATP synthesizing (state 3) mitochondria might contribute to oxidative stress or signaling.
Marcu R., Rapino S., Trinei M., Valenti G., Marcaccio M., Pelicci P.G., et al. (2012). Electrochemical study of hydrogen peroxide formation in isolated mitochondria. BIOELECTROCHEMISTRY, 85, 21-28 [10.1016/j.bioelechem.2011.11.005].
Electrochemical study of hydrogen peroxide formation in isolated mitochondria
RAPINO, STEFANIA;VALENTI, GIOVANNI;MARCACCIO, MASSIMO;PAOLUCCI, FRANCESCO;
2012
Abstract
The majority of methods, with exception of electron paramagnetic resonance, used to evaluate the identity, the rate and the conditions of the reactive oxygen species produced by mitochondria, are mainly based on oxidation sensitive markers. Following latest electrochemical methodology, we implemented a novel electrochemical assay for the investigation of aerobic metabolism in preparations of isolated mitochondria through simultaneous measurement of O-2 consumption and reactive species production. This electrochemical assay reveals active H2O2 production by respiring mouse liver mitochondria, and shows that ATP synthase activation and moderate depolarization increase the rate of H2O2 formation, suggesting that ATP synthesizing (state 3) mitochondria might contribute to oxidative stress or signaling.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
