OBJECTIVE: To investigate spindle behavior during and after slow freezing at room temperature (RT) and vitrification at different temperatures. DESIGN: Randomized, comparative study. SETTING: University hospital. PATIENT(S): Patients undergoing IVF treatment volunteered for the study and donated part of their supernumerary oocytes. INTERVENTION(S): Metaphase II oocytes were divided into group A: slow freezing RT /thawing RT; group B: vitrification RT/warming RT; group C: vitrification RT/warming 37 degrees C; and group D: vitrification 37 degrees C/warming 37 degrees C. Spindle presence was evaluated at each step of the four procedures and in culture. MAIN OUTCOME MEASURE(S): Cumulative spindle recovery rate comparing warming phase of the three vitrification groups and culture phase among the four groups. RESULT(S): During warming, the three vitrification groups showed a significantly fast spindle recovery rate compared to the thawing of the slow freezing group. A progressively significant fast cumulative recovery rate was observed in the three vitrification groups by increasing the number of phases at physiological temperature (hazard rate = 2.68; 95% confidence interval 1.71-4.02). CONCLUSION(S): The present study demonstrates that spindle recovery is faster in vitrification than in slow freezing. These data support a possible protective effect of vitrification/warming at 37 degrees C on the meiotic spindle structure and, therefore, on the subsequent clinical outcome of the procedure, although comparative clinical studies are needed.

Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing / Ciotti PM.; Porcu E.; Notarangelo L.; Magrini O.; Bazzocchi A.; Venturoli S.. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - STAMPA. - 91(6):(2009), pp. 2399-2407. [10.1016/j.fertnstert.2008.03.013]

Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing.

PORCU, ELEONORA;NOTARANGELO, LEONARDO;MAGRINI, OTELLO;VENTUROLI, STEFANO
2009

Abstract

OBJECTIVE: To investigate spindle behavior during and after slow freezing at room temperature (RT) and vitrification at different temperatures. DESIGN: Randomized, comparative study. SETTING: University hospital. PATIENT(S): Patients undergoing IVF treatment volunteered for the study and donated part of their supernumerary oocytes. INTERVENTION(S): Metaphase II oocytes were divided into group A: slow freezing RT /thawing RT; group B: vitrification RT/warming RT; group C: vitrification RT/warming 37 degrees C; and group D: vitrification 37 degrees C/warming 37 degrees C. Spindle presence was evaluated at each step of the four procedures and in culture. MAIN OUTCOME MEASURE(S): Cumulative spindle recovery rate comparing warming phase of the three vitrification groups and culture phase among the four groups. RESULT(S): During warming, the three vitrification groups showed a significantly fast spindle recovery rate compared to the thawing of the slow freezing group. A progressively significant fast cumulative recovery rate was observed in the three vitrification groups by increasing the number of phases at physiological temperature (hazard rate = 2.68; 95% confidence interval 1.71-4.02). CONCLUSION(S): The present study demonstrates that spindle recovery is faster in vitrification than in slow freezing. These data support a possible protective effect of vitrification/warming at 37 degrees C on the meiotic spindle structure and, therefore, on the subsequent clinical outcome of the procedure, although comparative clinical studies are needed.
2009
Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing / Ciotti PM.; Porcu E.; Notarangelo L.; Magrini O.; Bazzocchi A.; Venturoli S.. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - STAMPA. - 91(6):(2009), pp. 2399-2407. [10.1016/j.fertnstert.2008.03.013]
Ciotti PM.; Porcu E.; Notarangelo L.; Magrini O.; Bazzocchi A.; Venturoli S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/116390
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