Purpose. Hypothermic machine perfusion systems seem more effective than the current static storage to prevent cold ischemic liver injury. Thus, we test an innovative hyperbaric hypothermicmachine perfusion (HHMP), which combines hyperbaric oxygenation of the preservation solution and continuous perfusion of the graft. Methods. Rat livers were preserved with Celsior solution according to 4 different modalities: normobaric static preservation; hyperbaric static preservation at 2 atmosphere absolute (ATA); normobaric dynamic preservation, with continuous perfusion; hyperbaric dynamic preservation, with continuous perfusion at 2 ATA. After 24 h cold preservation, we assessed different parameters. Results. Compared to baseline, livers preserved with the current static storage showed severe ultrastructural damage, glycogen depletion and an increased oxidative stress. Normobaric perfused livers showed improved hepatocyte ultrastructure and ameliorated glycogen stores, but they still suffered a significant oxidative damage. The addition of hyperbaric oxygen produces an extra benefit by improving oxidative injury and by inducing endothelial NO synthase (eNOS) gene expression. Conclusions. Preservation by means of the present innovative HHMP reduced the liver injury occurring after the current static cold storage by lowering glycogen depletion and oxidative damage. Interestingly, only the use of hyperbaric oxygen was associated to a blunted oxidative stress and an increased eNOS gene expression.

Giannone FA, Trerè D, Domenicali M, Grattagliano I, Baracca A, Sgarbi G, et al. (2012). An innovative hyperbaric hypothermic machine perfusion protects the liver from experimental preservation injury. THE SCIENTIFIC WORLD JOURNAL, 2012, 1-9 [10.1100/2012/573410].

An innovative hyperbaric hypothermic machine perfusion protects the liver from experimental preservation injury

GIANNONE, FERDINANDO;TRERE', DAVIDE;DOMENICALI, MARCO;BARACCA, ALESSANDRA;SGARBI, GIANLUCA;MAGGIOLI, CATERINA;SOLAINI, GIANCARLO;DERENZINI, MASSIMO;BERNARDI, MAURO;CARACENI, PAOLO
2012

Abstract

Purpose. Hypothermic machine perfusion systems seem more effective than the current static storage to prevent cold ischemic liver injury. Thus, we test an innovative hyperbaric hypothermicmachine perfusion (HHMP), which combines hyperbaric oxygenation of the preservation solution and continuous perfusion of the graft. Methods. Rat livers were preserved with Celsior solution according to 4 different modalities: normobaric static preservation; hyperbaric static preservation at 2 atmosphere absolute (ATA); normobaric dynamic preservation, with continuous perfusion; hyperbaric dynamic preservation, with continuous perfusion at 2 ATA. After 24 h cold preservation, we assessed different parameters. Results. Compared to baseline, livers preserved with the current static storage showed severe ultrastructural damage, glycogen depletion and an increased oxidative stress. Normobaric perfused livers showed improved hepatocyte ultrastructure and ameliorated glycogen stores, but they still suffered a significant oxidative damage. The addition of hyperbaric oxygen produces an extra benefit by improving oxidative injury and by inducing endothelial NO synthase (eNOS) gene expression. Conclusions. Preservation by means of the present innovative HHMP reduced the liver injury occurring after the current static cold storage by lowering glycogen depletion and oxidative damage. Interestingly, only the use of hyperbaric oxygen was associated to a blunted oxidative stress and an increased eNOS gene expression.
2012
Giannone FA, Trerè D, Domenicali M, Grattagliano I, Baracca A, Sgarbi G, et al. (2012). An innovative hyperbaric hypothermic machine perfusion protects the liver from experimental preservation injury. THE SCIENTIFIC WORLD JOURNAL, 2012, 1-9 [10.1100/2012/573410].
Giannone FA; Trerè D; Domenicali M; Grattagliano I; Baracca A; Sgarbi G; Maggioli C; Longobardi P; Solaini G; Derenzini M; Bernardi M; Caraceni P....espandi
File in questo prodotto:
File Dimensione Formato  
Hyperbarism.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 7.43 MB
Formato Adobe PDF
7.43 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/115811
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 8
social impact