H9C2 interaction with PLLA nanofibrous scaffolds

A recent advance in tissue engineering is the development of biomimetic nanofiber for tissue regeneration. Using electrospinning nanotechnology, fibrous scaffolds can be fabricated in a controllable manner from solutions of many biocompatible polymers; the final result is a scaffold displaying a 3D architecture similar to that seen in the extracellular matrix natural microenvironment. By electrospinning it is possible to produce random or aligned nanofiber mats able to influence cell behaviour that can be potentially useful in tailored clinical applications. However, in the context of heart tissue repair, few studies are reported and it is not yet established whether these biomimetic nanofiber scaffolds nay have suitable properties for promoting cell adhesion, proliferation and migration. The aim of this work is to produce electrospun nanostructured polymeric bioresorbable and biocompatible scaffolds using poly-L-lactic acid (PLLA) and to investigate the cell/scaffold interactions using H9c2 myoblast cells derived from embryonic rat heart.