Anti-listerial activity of coatings entrapping living bacteria.

Polyvinyl alcohol (PVOH) based coatings entrapping either living bacteriocin-producer Enterococcus casseliflavus IM 416K1 bacteria or Enterocin 416K1 have been prepared and applied to poly(ethylene terephthalate) (PET) films. The antimicrobial activity of coated PET films was evaluated against Listeria monocytogenes NCTC 10888 by qualitative agar diffusion assays and by direct contact with artificially contaminated food samples (würstel and seasoned cheese) stored at 4 °C and 22 °C. Anti-listerial activity of both coatings was observed for both tests. However, the live-enterococcus doped coatings showed a much more remarkable anti-listerial activity than enterocin doped ones. Interestingly, live-enterococcus doped coatings lead to a strong decrease of L. monocytogenes viable counts even at 22 °C, indicating that they are able to contrast efficiently the fast L. monocytogenes growth occurring at this temperature in würstel samples. In this respect, they can be considered smart coatings, being able to be responsive towards an accidental rise of temperature during food storage. The capability of bacteria to survive for a long time can also assure a long lasting antibacterial activity. Coatings entrapping living Enterococcus casseliflavus IM 416K1 can produce Enterocin 416K1, a bacteriocin having a strong antibacterial activity towards Listeria monocytogenes . Such a kind of coating shows a long lasting antimicrobial activity and can behave like a smart coating, being able to be responsive to an accidental rise of temperature during food storage.