There is a need for fast, simple and reliable analytical methods for the analysis of fluoxetine and norfluoxetine in patients who voluntarily or involuntarily have taken an overdose of the drug. A new liquid chromatographic method with diode array detection is presented herein for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma for toxicological purposes. A mobile phase composed of acetonitrile and aqueous tetramethylammonium perchlorate allows to obtain the complete separation of the analytes on a C18 reversed phase column. The fast and accurate sample pre-treatment step is carried out by means of solid-phase extraction using hydrophilic-lipophilic balance cartridges and loading 100 µL of plasma only. This procedure gives satisfactory extraction yield values, as well as good plasma sample purification from matrix interference. Linearity was obtained in the 150-3000 ng/mL range for both analytes. Selectivity with respect to other psychotropic drugs was satisfactory. The method seems to be suitable for the analysis of fluoxetine and its metabolite in human plasma for depressed patients in overdose.

C. Sabbioni, F. Bugamelli, G. Varani, L. Mercolini, A. Musenga, M.A. Saracino, et al. (2004). A rapid HPLC-DAD method for the analysis of fluoxetine and norfluoxetine in plasma from overdose patients. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 36, 351-356 [10.1016/j.jpba.2004.06.008].

A rapid HPLC-DAD method for the analysis of fluoxetine and norfluoxetine in plasma from overdose patients

MERCOLINI, LAURA;RAGGI, MARIA AUGUSTA
2004

Abstract

There is a need for fast, simple and reliable analytical methods for the analysis of fluoxetine and norfluoxetine in patients who voluntarily or involuntarily have taken an overdose of the drug. A new liquid chromatographic method with diode array detection is presented herein for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma for toxicological purposes. A mobile phase composed of acetonitrile and aqueous tetramethylammonium perchlorate allows to obtain the complete separation of the analytes on a C18 reversed phase column. The fast and accurate sample pre-treatment step is carried out by means of solid-phase extraction using hydrophilic-lipophilic balance cartridges and loading 100 µL of plasma only. This procedure gives satisfactory extraction yield values, as well as good plasma sample purification from matrix interference. Linearity was obtained in the 150-3000 ng/mL range for both analytes. Selectivity with respect to other psychotropic drugs was satisfactory. The method seems to be suitable for the analysis of fluoxetine and its metabolite in human plasma for depressed patients in overdose.
2004
C. Sabbioni, F. Bugamelli, G. Varani, L. Mercolini, A. Musenga, M.A. Saracino, et al. (2004). A rapid HPLC-DAD method for the analysis of fluoxetine and norfluoxetine in plasma from overdose patients. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 36, 351-356 [10.1016/j.jpba.2004.06.008].
C. Sabbioni; F. Bugamelli; G. Varani; L. Mercolini; A. Musenga; M.A. Saracino; S. Fanali; M.A. Raggi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/11144
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 48
  • ???jsp.display-item.citation.isi??? 46
social impact