In this study, a forward-genetics analysis was performed on a portion of TILLMore, a chemically mutagenized population of barley cv. ‘Morex’ (http://www.distagenomics.unibo.it/TILLMore/), to identify root morphology alterations by comparison with ‘Morex’ wild-type. For this purpose, a simple paper-roll approach was performed to identify phenotypic variants at the seedling stage. The analysis of c. 1000 M4 families allowed us to identify c. 70 lines with altered root morphology. A more accurate phenotypic characterization of a portion of the mutant lines has been performed using stereomicroscopy and a scanning electron microscopy approach.
Identifiction of root morphology mutants in barley / BOVINA R.; TALAME' V.; FERRI M.; TUBEROSA R.; CHMIELEWSKA B.; SZAREJKO I.; SANGUINETI M.C.. - In: PLANT GENETIC RESOURCES. - ISSN 1479-2621. - STAMPA. - 9:(2011), pp. 357-360. [10.1017/S1479262111000529]
Identifiction of root morphology mutants in barley
BOVINA, RICCARDO;TALAME', VALENTINA;FERRI, MATTEO;TUBEROSA, ROBERTO;SANGUINETI, MARIA CORINNA
2011
Abstract
In this study, a forward-genetics analysis was performed on a portion of TILLMore, a chemically mutagenized population of barley cv. ‘Morex’ (http://www.distagenomics.unibo.it/TILLMore/), to identify root morphology alterations by comparison with ‘Morex’ wild-type. For this purpose, a simple paper-roll approach was performed to identify phenotypic variants at the seedling stage. The analysis of c. 1000 M4 families allowed us to identify c. 70 lines with altered root morphology. A more accurate phenotypic characterization of a portion of the mutant lines has been performed using stereomicroscopy and a scanning electron microscopy approach.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
