Escherichia coli, Campylobacter jejuni, Pseudomonas aeuroginosa and Staphylococcus aureus are food-borne pathogenic bacteria and renowned agents of related intestinal diseases. All these species exploit their pathogenesis through the production of toxins. In particular, the Cytolethal distending toxins (CDT) of both C. jejuni and E.coli are potent inhibitor of G2/M phase of intestinal enterocytes, while Pyocianin of P. aeuroginosa and Enterotoxin of S. aureus are agents of food poisoning. In this study, we have analysed the antimicrobial activity of essential oils obtained from different varieties of Monarda fistulosa L. against Entero-pathogenic E. coli, C. jejuni, S. aureus and P. aeruginosa. The approach is that of Minimal inhibitory Concentration (MIC) analysis, using the micro broth dilution method, in association with the study of gene expression profiles of toxins production, through the ΔΔCt assay and Fast- SYBR Green I chemistry, using Real Time PCR. The aim is that to determine the highest MIC that does not inhibit bacterial growth, but down-regulates toxins expression. Considering the toxins that were analysed with the expression of their encoding genes, we choose Cytolethal distending toxin (CDT) for both E. coli and C. jejuni, Pyocyanin for P. aeuroginosa and Enterotoxin for S. aureus.
WILD BERGAMOT ESSENTIAL OIL INHIBITS THE GROWTH OF FOOD-BORNE PATHOGENS AND DOWNREGULATES THE EXPRESSION OF RELATED TOXIN-ENCODING GENES / Nissen L.; Arora K.; Santini C.; Mattarelli P.; Bellardi M.G.; Sgorbati B.; Biavati B.. - In: GUT. - ISSN 0017-5749. - STAMPA. - 60 (Suppl. 3):(2011), pp. A209-A209. (Intervento presentato al convegno 19th UEGW Stockolm 2011 tenutosi a Stockolm nel 22-26 October 2011).
WILD BERGAMOT ESSENTIAL OIL INHIBITS THE GROWTH OF FOOD-BORNE PATHOGENS AND DOWNREGULATES THE EXPRESSION OF RELATED TOXIN-ENCODING GENES.
NISSEN, LORENZO;SANTINI, CECILIA;MATTARELLI, PAOLA;BELLARDI, MARIA GRAZIA;SGORBATI, BARBARA;BIAVATI, BRUNO
2011
Abstract
Escherichia coli, Campylobacter jejuni, Pseudomonas aeuroginosa and Staphylococcus aureus are food-borne pathogenic bacteria and renowned agents of related intestinal diseases. All these species exploit their pathogenesis through the production of toxins. In particular, the Cytolethal distending toxins (CDT) of both C. jejuni and E.coli are potent inhibitor of G2/M phase of intestinal enterocytes, while Pyocianin of P. aeuroginosa and Enterotoxin of S. aureus are agents of food poisoning. In this study, we have analysed the antimicrobial activity of essential oils obtained from different varieties of Monarda fistulosa L. against Entero-pathogenic E. coli, C. jejuni, S. aureus and P. aeruginosa. The approach is that of Minimal inhibitory Concentration (MIC) analysis, using the micro broth dilution method, in association with the study of gene expression profiles of toxins production, through the ΔΔCt assay and Fast- SYBR Green I chemistry, using Real Time PCR. The aim is that to determine the highest MIC that does not inhibit bacterial growth, but down-regulates toxins expression. Considering the toxins that were analysed with the expression of their encoding genes, we choose Cytolethal distending toxin (CDT) for both E. coli and C. jejuni, Pyocyanin for P. aeuroginosa and Enterotoxin for S. aureus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
