GLUTs are a family of proteins that facilitate the transport of glucose and other hexoses through the plasma membrane of the cells. GLUTs are present in mammalian spermatozoon's membrane in different isoforms and they supply metabolic substrates for all the cell's activities such as motility, homoeostasis and fertilization. As studies about donkey spermatozoa and their metabolism are lacking, this study was aimed at detecting GLUTs 1, 2, 3, and 5 presence by western blotting technique and at determining their localization on the plasma membrane by indirect immunofluoresence. Each protein showed a typical localization on the sperm cells' plasma membrane, differencing the one to the other on the basis of the hexose they transport. We also highlighted some differences between GLUTs distribution and molecular weight in donkey spermatozoa and its nearest relative, the horse.

Detection and localization of GLUTs 1,2,3 and 5 in donkey spermatozoa

BUCCI, DIEGO;SPINACI, MARCELLA;VALLORANI, CLAUDIA;ISANI, GLORIA;TAMANINI, CARLO;GALEATI, GIOVANNA
2010

Abstract

GLUTs are a family of proteins that facilitate the transport of glucose and other hexoses through the plasma membrane of the cells. GLUTs are present in mammalian spermatozoon's membrane in different isoforms and they supply metabolic substrates for all the cell's activities such as motility, homoeostasis and fertilization. As studies about donkey spermatozoa and their metabolism are lacking, this study was aimed at detecting GLUTs 1, 2, 3, and 5 presence by western blotting technique and at determining their localization on the plasma membrane by indirect immunofluoresence. Each protein showed a typical localization on the sperm cells' plasma membrane, differencing the one to the other on the basis of the hexose they transport. We also highlighted some differences between GLUTs distribution and molecular weight in donkey spermatozoa and its nearest relative, the horse.
Bucci D.; Spinaci M.; Vallorani C.; Contri A.; Carluccio A.; Isani G.; Tamanini C.; Galeati G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/103510
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