TILLING in barley: The TILLMore resource

TILLING (Targeting Induced Local Lesion IN Genomes) is a reverse-genetics approach combining chemical mutagenesis with a sensitive DNA screening-technique to identify point mutations in target genes (McCallum et al. 2000). We developed TILLMore, a TILLING resource in barley (cv. Morex) including 4,906 M4 families derived from a sodium azide treatment. Molecular screening for mutation at agronomical important genes was based on the analysis of 8- to 12-fold DNA pools produced from M2 or M3 DNA samples. The mutagenized population, although developed for reverse-genetics purposes, was also analysed for forward-genetics studies.