The sodium/iodide symporter (NIS) is primarily responsible for iodide accumulation in the thyroid gland for the synthesis of thyroid hormones; however, it can also transport other lyotropic anions in the thyroid gland and nonthyroid tissues. Some NIS substrates have important physiological or clinical roles, and others are environmental contaminants with health-related consequences. The aim of this study was to assess the utility of a yellow fluorescent protein variant, YFP-H148Q/I152L, as a biosensor to monitor the cellular uptake of NIS substrates, including thiocyanate (SCN-), nitrate (NO3-), chlorate (ClO3-), perchlorate (ClO4-), and perrhenate (ReO4-). The fluorescence of purified YFP-H148Q/I152L was suppressed by anions with an order of potency of ReO4- > ClO4- = I- = SCN- = ClO3- > NO3- ≫ Cl-. Anions also suppressed the fluorescence of YFP-H148Q/I152L expressed in FRTL-5, a thyroid cell line with high NIS expression. Quantitation of intracellular concentrations revealed differences among anions in the affinity and maximal velocity of NIS-mediated uptake as well as in the rate constant for passive efflux. These results suggest that YFP-H148Q/I152L can serve as an intracellular biosensor of NIS-transported anions and may be useful to study the physiology of endogenous anions as well as the health-related consequences of environmental anions.
Di Bernardo J., Iosco C., Rhoden K.J. (2011). Intracellular anion fluorescence assay for sodium/iodide symporter substrates. ANALYTICAL BIOCHEMISTRY, 415, 32-38 [10.1016/j.ab.2011.04.017].
Intracellular anion fluorescence assay for sodium/iodide symporter substrates
DI BERNARDO, JULIE;IOSCO, CARMELA;RHODEN, KERRY JANE
2011
Abstract
The sodium/iodide symporter (NIS) is primarily responsible for iodide accumulation in the thyroid gland for the synthesis of thyroid hormones; however, it can also transport other lyotropic anions in the thyroid gland and nonthyroid tissues. Some NIS substrates have important physiological or clinical roles, and others are environmental contaminants with health-related consequences. The aim of this study was to assess the utility of a yellow fluorescent protein variant, YFP-H148Q/I152L, as a biosensor to monitor the cellular uptake of NIS substrates, including thiocyanate (SCN-), nitrate (NO3-), chlorate (ClO3-), perchlorate (ClO4-), and perrhenate (ReO4-). The fluorescence of purified YFP-H148Q/I152L was suppressed by anions with an order of potency of ReO4- > ClO4- = I- = SCN- = ClO3- > NO3- ≫ Cl-. Anions also suppressed the fluorescence of YFP-H148Q/I152L expressed in FRTL-5, a thyroid cell line with high NIS expression. Quantitation of intracellular concentrations revealed differences among anions in the affinity and maximal velocity of NIS-mediated uptake as well as in the rate constant for passive efflux. These results suggest that YFP-H148Q/I152L can serve as an intracellular biosensor of NIS-transported anions and may be useful to study the physiology of endogenous anions as well as the health-related consequences of environmental anions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
