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EnglishRecent progress in inkjet printing of parts of biosensors are highlighted, with particular reference to the printing of biologically active molecules. We describe a system constituted by a thermal inkjet printer, adapted to layering a bidimensional array of dots [701 701 dots per inch] on solid supports. The printer was used to depose a β-galactosidase (GAL)-containing ink on a polyester sheet, with dots obtained from 10 pL drops, each drop containing in turn 6 pg of enzyme. The activity of GAL after the preparation was determined using a colorimetric probe (Brilliant Blue FCF). The activity loss of the microdeposed enzymes was found to be around 15%, showing that the 2 µsec-lasting thermal shock experienced by the biomolecule into the printhead nozzle affects to a lesser extent the activity of the thermal inkjet deposited enzyme. In conclusion, the most recent findings of our group in this line are depicted, and a view of possible future developments of the “biopolytronics” field is outlined.
| Titolo: | Thermal Inkjet Technology for the Microdeposition of Biological Molecules as a Viable Route for the Realization of Biosensors |
| Autore/i: | SETTI, LEONARDO; PIANA, CHIARA; BONAZZI, STEFANIA; BALLARIN, BARBARA; FRASCARO, DAVIDE; A. Fraleoni Morgera; GIULIANI, SILVIA |
| Autore/i Unibo: | |
| Anno: | 2004 |
| Rivista: | |
| Digital Object Identifier (DOI): | 10.1081/AL-120037587 |
| Abstract: | Recent progress in inkjet printing of parts of biosensors are highlighted, with particular reference to the printing of biologically active molecules. We describe a system constituted by a thermal inkjet printer, adapted to layering a bidimensional array of dots [701 701 dots per inch] on solid supports. The printer was used to depose a β-galactosidase (GAL)-containing ink on a polyester sheet, with dots obtained from 10 pL drops, each drop containing in turn 6 pg of enzyme. The activity of GAL after the preparation was determined using a colorimetric probe (Brilliant Blue FCF). The activity loss of the microdeposed enzymes was found to be around 15%, showing that the 2 µsec-lasting thermal shock experienced by the biomolecule into the printhead nozzle affects to a lesser extent the activity of the thermal inkjet deposited enzyme. In conclusion, the most recent findings of our group in this line are depicted, and a view of possible future developments of the “biopolytronics” field is outlined. |
| Data prodotto definitivo in UGOV: | 2005-10-05 19:43:53 |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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http://hdl.handle.net/11585/10231
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