Electrogeneratedchemiluminescence(ECL)bioassayhaswitnessed remarkablegrowthinbiosensinganddiagnostics.Anunderstandingofthe reactionmechanismwithinECLisimportantforimprovinganddesigning sensitiveECLmethods.Herein,asidelongECLmicroscopyandfiniteelement simulationaredevelopedfortomographyimagingfromsinglemagnetic microbeadsanddecipheringreactionmechanismwithinbead-based coreactantECLsystem.Rutheniumderivativelabeledmagneticbeads (Ru1-Mag@MB)andtri-n-propylaminearechosenastypicalcoreactantECL systems.ECLemissionsfromRu1-Mag@MBindifferentconditionsare imagedviathesidelongECLmicroscopy,whichcanallowthespatially resolvedmeasurementoftheECLemittinglayerfromsinglemicrobeadsin theverticaldirectionoftheelectrodesurface.Surface-confinedECLemissions aremainlyobservedatorneartheinterfacebetweentheelectrodesurfaceand theRu1-Mag@MB.ThesurfacedistributionplotsofECLintensity,and tomographyECL,acrosssingleRu1-Mag@MBdisplay“firstincreaseandthen decrease”phenomena.TheECLintensityandemissionextensionarevaried withthechangeoftheradicallifetime,thekineticsofchemicalreactions,and theopticalparametersofmicrobeads.ThesidelongECLmicroscopywiththe tomographyoftheECLemittinglayercanprovideapromisingwayfor insightfulmechanisticinformationwithinabead-basedECLsystem.
Feng, Y., Wang, C., Zhou, W., Yang, X., Paolucci, F., Valenti, G., et al. (2025). Tomography Electrogenerated Chemiluminescence Imaging from Magnetic Microbeads. SMALL, 21(22), 2500804-2500812 [10.1002/smll.202500804].
Tomography Electrogenerated Chemiluminescence Imaging from Magnetic Microbeads
Paolucci, Francesco;Valenti, Giovanni
;
2025
Abstract
Electrogeneratedchemiluminescence(ECL)bioassayhaswitnessed remarkablegrowthinbiosensinganddiagnostics.Anunderstandingofthe reactionmechanismwithinECLisimportantforimprovinganddesigning sensitiveECLmethods.Herein,asidelongECLmicroscopyandfiniteelement simulationaredevelopedfortomographyimagingfromsinglemagnetic microbeadsanddecipheringreactionmechanismwithinbead-based coreactantECLsystem.Rutheniumderivativelabeledmagneticbeads (Ru1-Mag@MB)andtri-n-propylaminearechosenastypicalcoreactantECL systems.ECLemissionsfromRu1-Mag@MBindifferentconditionsare imagedviathesidelongECLmicroscopy,whichcanallowthespatially resolvedmeasurementoftheECLemittinglayerfromsinglemicrobeadsin theverticaldirectionoftheelectrodesurface.Surface-confinedECLemissions aremainlyobservedatorneartheinterfacebetweentheelectrodesurfaceand theRu1-Mag@MB.ThesurfacedistributionplotsofECLintensity,and tomographyECL,acrosssingleRu1-Mag@MBdisplay“firstincreaseandthen decrease”phenomena.TheECLintensityandemissionextensionarevaried withthechangeoftheradicallifetime,thekineticsofchemicalreactions,and theopticalparametersofmicrobeads.ThesidelongECLmicroscopywiththe tomographyoftheECLemittinglayercanprovideapromisingwayfor insightfulmechanisticinformationwithinabead-basedECLsystem.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
