Modified Methylation Following Electrostimulation in a Standardized Setting - Complementing a Transcriptomic Analysis

Electrical stimulation (ES) is widely employed in both clinical therapies and research settings where it has shown promise in promoting tissue regeneration, wound healing and also inflammation control. Research has also highlighted ES as a regulator of DNA demethylation, which plays a critical role in nerve regeneration and cellular repair mechanisms. While the impact of ES on epigenetic processes is recognized, its broader effects on cellular functions, particularly in inflammation and wound healing, are less understood. We recently showed how ES impacts on inflammatory states by modulating transcriptomic and metabolomic profiles in a 3D in vitro model where human fibroblasts and keratinocytes are included in a collagen matrix, i.e even in the absence of nervous system. Here we propose to deepen our exploration on the differential effects on DNA methylation, including investigation of potential age acceleration with the usage of an epigenetic clock. These results support the potential of 5V Direct Current stimuli observed earlier at the transcriptional level to influence inflammatory processes, and suggest an involvement of DC5 in the control of senescent processes associated with inflammation.