Pathways and composition of dissolved organic carbon in a small agricultural catchment during base flow conditions

The amount and composition of organic carbon are major controls on water quality and ecological processes in streams. In this study we explored the fate of the quantity of dissolved organic carbon (DOC) and the composition of dissolved organic matter (DOM) in an agricultural hillslope - stream network system. We conducted our study in the 66 ha HOAL (Hydrological Open Air Laboratory) in Lower Austria. We measured DOC of the soil eluates from different land use units, water samples from the stream and from seven tributaries, and estimated DOM components by fluorescent spectrophotometry and PARAFAC analyses. Soil DOC shows the highest concentrations in summer, but DOC concentrations in the tributaries are lower in summer than in winter by between 19% and 31%. DOM composition of the soil eluate differs between land use units. The forest site exhibits the largest fractions of humic-like fluorophores and less labile DOM. DOM composition in the tributaries is, in addition to DOC, controlled by soil moisture. We estimated the DOC import from the tributaries into the stream as 125 kg during base flow conditions in the period February to December 2017 and the instream DOC production as 38 kg, considering mass balance and exchange with groundwater. Six out of seven DOM components have a positive net production along the stream, only aliphatic DOM with low molecular weight is consumed (65 % of its input). These findings suggest that agricultural land use increases DOC input into streams and alters their DOM quality. Instream processes modify DOM quality over short distances