n the present study, the employment of clone PClO inveterinary oncology has been investigated, with regard toimmunoreactivity in dogs and cats and reproducibility of themethod on samples with known and unknown time of fix-ation as often happens in stored material for retrospectiveinvestigations. The percentage of labeled nuclei (PCNA in-dex) was evaluated by computerized image analysis. Twodifferent values of PCNA index were considered: one count-ing as positive only the strongly labeled nuclei (SP-PCNAindex) and the other counting as positive all the labeled nuclei(TP-PCNA index). Both values of PCNA index were com-pared with mitotic index (number of mitoses per 1,000 cells)
Sarli, G., Benazzi, C., Preziosi, R., Marcato, P.S. (1995). Assessment of proliferative activity by anti-PCNA monoclonal antibodies in formalin-fixed, paraffin-embedded samples and correlation with mitotic index. VETERINARY PATHOLOGY, 32(1), 93-96 [10.1177/030098589503200120].
Assessment of proliferative activity by anti-PCNA monoclonal antibodies in formalin-fixed, paraffin-embedded samples and correlation with mitotic index
Sarli G.
;Benazzi C.;Preziosi R.;Marcato P. S.
1995
Abstract
n the present study, the employment of clone PClO inveterinary oncology has been investigated, with regard toimmunoreactivity in dogs and cats and reproducibility of themethod on samples with known and unknown time of fix-ation as often happens in stored material for retrospectiveinvestigations. The percentage of labeled nuclei (PCNA in-dex) was evaluated by computerized image analysis. Twodifferent values of PCNA index were considered: one count-ing as positive only the strongly labeled nuclei (SP-PCNAindex) and the other counting as positive all the labeled nuclei(TP-PCNA index). Both values of PCNA index were com-pared with mitotic index (number of mitoses per 1,000 cells)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
