In this study we evaluated the humoral response in 43 serum samples obtained from 15 patients suffering from Lyme borreliosis erythema migrans. A skin punch biopsy was obtained from each patient when entering the study and cultivated in BSKII medium. All the 15 cultures showed a positivity for borreliae within one month: 12 were identified as Borrelia afzelii, and the remaining 3 were B. garinii, as defined by PCR assay. The IgM response was identified by immunofluorescence assay (IFA) in 39/43 sera, whereas 37/43 sera were IgG positive. Using Western blot technique all the samples were reactive, both for IgM and IgG antibodies.
Marangoni, A., Sambri, V., Cimmino, C., Caruso, G., Mondardini, V., Cevenini, R. (1999). Evaluation of the immune response in culture-confirmed Lyme borreliosis erythema migrans patients. ZENTRALBLATT FUR BAKTERIOLOGIE, 289(5-7), 736-739 [10.1016/S0934-8840(99)80045-0].
Evaluation of the immune response in culture-confirmed Lyme borreliosis erythema migrans patients
Marangoni A.;Sambri V.;Cevenini R.
1999
Abstract
In this study we evaluated the humoral response in 43 serum samples obtained from 15 patients suffering from Lyme borreliosis erythema migrans. A skin punch biopsy was obtained from each patient when entering the study and cultivated in BSKII medium. All the 15 cultures showed a positivity for borreliae within one month: 12 were identified as Borrelia afzelii, and the remaining 3 were B. garinii, as defined by PCR assay. The IgM response was identified by immunofluorescence assay (IFA) in 39/43 sera, whereas 37/43 sera were IgG positive. Using Western blot technique all the samples were reactive, both for IgM and IgG antibodies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
