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EnglishBeet necrotic yellow vein virus (BNYVV), the causal agent of Rhizomania, is the model species of Benyvirus genus together with Beet soil-borne mosaic virus (BSBMV). Both viruses are vectored by Polymyxa betae. By the use of full-length clones from all genomic RNAs, molecular interactions between plant and Benyviruses are being investigated exploiting biological, epidemiological and molecular similarities/divergences between BNYVV and BSBMV. Results. cDNA copies from all BSBMV RNAs and BNYVV (type P) RNA-1 and 2 have been successfully synthesized and infectivity of the in vitro transcribed RNAs evaluated through rub-inoculation onto Chenopodium quinoa plant leaves. BSBMV RNA2 and BNYVV (type P) RNA-1 cDNA clones are not infectious and still need to be modified. A molecular characterization of chimeric strain consisting of B-type RNA-1 and P-type RNA-2 in comparison with B-type RNA-1 and RNA-2 will be reported. Difference on the expression level of proteins encoded by BNYVV P/B-type RNA-3 (p25), RNA-4 (p31) and RNA-5 (p26) have been evidenced.
| Titolo: | Sugar beet infecting benyviruses: mechanisms involved in the virus-host interaction | |
| Autore/i: | D'ALONZO, MASSIMILIANO; C. Weidemann; D. Gilmer; RUBIES AUTONELL, CONCEPCION; RATTI, CLAUDIO | |
| Autore/i Unibo: | ||
| Anno: | 2010 | |
| Titolo del libro: | European Society for Virology | |
| Pagina iniziale: | 281 | |
| Pagina finale: | 281 | |
| Abstract: | Beet necrotic yellow vein virus (BNYVV), the causal agent of Rhizomania, is the model species of Benyvirus genus together with Beet soil-borne mosaic virus (BSBMV). Both viruses are vectored by Polymyxa betae. By the use of full-length clones from all genomic RNAs, molecular interactions between plant and Benyviruses are being investigated exploiting biological, epidemiological and molecular similarities/divergences between BNYVV and BSBMV. Results. cDNA copies from all BSBMV RNAs and BNYVV (type P) RNA-1 and 2 have been successfully synthesized and infectivity of the in vitro transcribed RNAs evaluated through rub-inoculation onto Chenopodium quinoa plant leaves. BSBMV RNA2 and BNYVV (type P) RNA-1 cDNA clones are not infectious and still need to be modified. A molecular characterization of chimeric strain consisting of B-type RNA-1 and P-type RNA-2 in comparison with B-type RNA-1 and RNA-2 will be reported. Difference on the expression level of proteins encoded by BNYVV P/B-type RNA-3 (p25), RNA-4 (p31) and RNA-5 (p26) have been evidenced. | |
| Data prodotto definitivo in UGOV: | 22-feb-2011 | |
| Appare nelle tipologie: | 4.02 Riassunto (Abstract) |
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