Beet necrotic yellow vein virus (BNYVV), the causal agent of Rhizomania, is the model species of Benyvirus genus together with Beet soil-borne mosaic virus (BSBMV). Both viruses are vectored by Polymyxa betae. By the use of full-length clones from all genomic RNAs, molecular interactions between plant and Benyviruses are being investigated exploiting biological, epidemiological and molecular similarities/divergences between BNYVV and BSBMV. Results. cDNA copies from all BSBMV RNAs and BNYVV (type P) RNA-1 and 2 have been successfully synthesized and infectivity of the in vitro transcribed RNAs evaluated through rub-inoculation onto Chenopodium quinoa plant leaves. BSBMV RNA2 and BNYVV (type P) RNA-1 cDNA clones are not infectious and still need to be modified. A molecular characterization of chimeric strain consisting of B-type RNA-1 and P-type RNA-2 in comparison with B-type RNA-1 and RNA-2 will be reported. Difference on the expression level of proteins encoded by BNYVV P/B-type RNA-3 (p25), RNA-4 (p31) and RNA-5 (p26) have been evidenced.

Sugar beet infecting benyviruses: mechanisms involved in the virus-host interaction

D'ALONZO, MASSIMILIANO;RUBIES AUTONELL, CONCEPCION;RATTI, CLAUDIO
2010

Abstract

Beet necrotic yellow vein virus (BNYVV), the causal agent of Rhizomania, is the model species of Benyvirus genus together with Beet soil-borne mosaic virus (BSBMV). Both viruses are vectored by Polymyxa betae. By the use of full-length clones from all genomic RNAs, molecular interactions between plant and Benyviruses are being investigated exploiting biological, epidemiological and molecular similarities/divergences between BNYVV and BSBMV. Results. cDNA copies from all BSBMV RNAs and BNYVV (type P) RNA-1 and 2 have been successfully synthesized and infectivity of the in vitro transcribed RNAs evaluated through rub-inoculation onto Chenopodium quinoa plant leaves. BSBMV RNA2 and BNYVV (type P) RNA-1 cDNA clones are not infectious and still need to be modified. A molecular characterization of chimeric strain consisting of B-type RNA-1 and P-type RNA-2 in comparison with B-type RNA-1 and RNA-2 will be reported. Difference on the expression level of proteins encoded by BNYVV P/B-type RNA-3 (p25), RNA-4 (p31) and RNA-5 (p26) have been evidenced.
European Society for Virology
281
281
M. D’Alonzo; C. Weidemann; D. Gilmer; C. Rubies Autonell; C. Ratti.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/99300
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