Since the nineteen’s, when the first outbreak of grapevine yellows (GY) in Tuscany was reported, field inspections and laboratory analysis were conducted. However, regular surveys of Flavescence dorée (FD), Bois doir (BN) presence were carried out in Tuscany since 2003. Several regional and non regional public institutions were involved in a control strategy aimed to the containment of GY spreading in the region. During the last years and in strength collaborations among the different institutions, activities ranging from: (i) annual survey through extensive field inspections in vineyards from the ten provinces of the Tuscany region; (ii) collection of samples showing symptoms referable to GY diseases; (iii) application and implementation of molecular techniques for detection and characterization of phytoplasma ribosomal subgroups associated with the disease were carried out. Nested-PCR, PCR/RLFP and Real-time PCR were applied as main laboratory techniques. Often, particularly interesting samples were exchanged among partners and analyzed with different techniques for a final phytoplasma identification and strain characterization. Samples collected and tested with molecular analyses were 3,453, among them 2,304 (66,7%) resulted positive to phytoplasma associated with GY. In details, BN was largely the prevalent GY in Tuscany with an overall incidence of 95.7% (2,205 out of 2,304). The presence of FD was verified in 99 samples distributed in five provinces: Massa-Carrara (59), Florence (12), Lucca (11), Siena (10) and Arezzo (7).

Grapevine yellows in Tuscany: results of a seven years survey.

PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2010

Abstract

Since the nineteen’s, when the first outbreak of grapevine yellows (GY) in Tuscany was reported, field inspections and laboratory analysis were conducted. However, regular surveys of Flavescence dorée (FD), Bois doir (BN) presence were carried out in Tuscany since 2003. Several regional and non regional public institutions were involved in a control strategy aimed to the containment of GY spreading in the region. During the last years and in strength collaborations among the different institutions, activities ranging from: (i) annual survey through extensive field inspections in vineyards from the ten provinces of the Tuscany region; (ii) collection of samples showing symptoms referable to GY diseases; (iii) application and implementation of molecular techniques for detection and characterization of phytoplasma ribosomal subgroups associated with the disease were carried out. Nested-PCR, PCR/RLFP and Real-time PCR were applied as main laboratory techniques. Often, particularly interesting samples were exchanged among partners and analyzed with different techniques for a final phytoplasma identification and strain characterization. Samples collected and tested with molecular analyses were 3,453, among them 2,304 (66,7%) resulted positive to phytoplasma associated with GY. In details, BN was largely the prevalent GY in Tuscany with an overall incidence of 95.7% (2,205 out of 2,304). The presence of FD was verified in 99 samples distributed in five provinces: Massa-Carrara (59), Florence (12), Lucca (11), Siena (10) and Arezzo (7).
XVI Convegno Nazionale SIPaV
15
15
Rizzo D.; S. Paltrinieri; H. Bouyahia; P. Braccini; M. Della Bartola; C. Milano; A. Materazzi; A. Bertaccini.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/98474
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact