Capillary zone electrophoresis (CZE) is a techniques suitable for separation and characterization of humic (HS) and humic-like substances (HLS) because their polyelectrolytic nature, although their application was limited because artifacts or interactions among HSs and other chemicals occur during the electrophoretic run. Two different buffers have been used to analyze the HS with CZE: (i) a non-interactive alkaline buffer (i.e. carbonate or phosphate) whose pattern show broad bands, useful to calculate the average electrophoretic mobility (AEM) of the HS and (ii) buffers with interactive agents (i.e. borate, urea, SDS, EDTA) that, separating several peaks or bands, permit a “finger-print” characterization of HSs. In the present paper we report some results about the characterization of peat humic acids (HA) fractions obtained by tangential ultra-filtration by CZE in order to improve the knowledge about electrophoretic behavior of HS and the application of CZE to the analysis of organic matter extracted from organic fertilizers.

Capillary Zone Electrophoresis (CZE) Characterization of Peat Humic Acids Fractions Obtained by Tangential Ultra-Filtration

CAVANI, LUCIANO;CIAVATTA, CLAUDIO
2010

Abstract

Capillary zone electrophoresis (CZE) is a techniques suitable for separation and characterization of humic (HS) and humic-like substances (HLS) because their polyelectrolytic nature, although their application was limited because artifacts or interactions among HSs and other chemicals occur during the electrophoretic run. Two different buffers have been used to analyze the HS with CZE: (i) a non-interactive alkaline buffer (i.e. carbonate or phosphate) whose pattern show broad bands, useful to calculate the average electrophoretic mobility (AEM) of the HS and (ii) buffers with interactive agents (i.e. borate, urea, SDS, EDTA) that, separating several peaks or bands, permit a “finger-print” characterization of HSs. In the present paper we report some results about the characterization of peat humic acids (HA) fractions obtained by tangential ultra-filtration by CZE in order to improve the knowledge about electrophoretic behavior of HS and the application of CZE to the analysis of organic matter extracted from organic fertilizers.
Advances in Natural Organic Matter and Humic Substances Research 2008-2010 (Vol. 1, 2 & 3), Proceedings Book of the Communications presented to the 15th Meeting of the International Humic Substances Society
139
142
L. Cavani; C. Ciavatta
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/98334
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