Capillary zone electrophoresis (CZE) is a techniques suitable for separation and characterization of humic (HS) and humic-like substances (HLS) because their polyelectrolytic nature, although their application was limited because artifacts or interactions among HSs and other chemicals occur during the electrophoretic run. Two different buffers have been used to analyze the HS with CZE: (i) a non-interactive alkaline buffer (i.e. carbonate or phosphate) whose pattern show broad bands, useful to calculate the average electrophoretic mobility (AEM) of the HS and (ii) buffers with interactive agents (i.e. borate, urea, SDS, EDTA) that, separating several peaks or bands, permit a “finger-print” characterization of HSs. In the present paper we report some results about the characterization of peat humic acids (HA) fractions obtained by tangential ultra-filtration by CZE in order to improve the knowledge about electrophoretic behavior of HS and the application of CZE to the analysis of organic matter extracted from organic fertilizers.
Capillary Zone Electrophoresis (CZE) Characterization of Peat Humic Acids Fractions Obtained by Tangential Ultra-Filtration / L. Cavani; C. Ciavatta. - ELETTRONICO. - 2:(2010), pp. 139-142. (Intervento presentato al convegno 15th Meeting of the International Humic Substances Society tenutosi a Tenerife - Canary Islands (Spain) nel June 27- July 2, 2010).
Capillary Zone Electrophoresis (CZE) Characterization of Peat Humic Acids Fractions Obtained by Tangential Ultra-Filtration
CAVANI, LUCIANO;CIAVATTA, CLAUDIO
2010
Abstract
Capillary zone electrophoresis (CZE) is a techniques suitable for separation and characterization of humic (HS) and humic-like substances (HLS) because their polyelectrolytic nature, although their application was limited because artifacts or interactions among HSs and other chemicals occur during the electrophoretic run. Two different buffers have been used to analyze the HS with CZE: (i) a non-interactive alkaline buffer (i.e. carbonate or phosphate) whose pattern show broad bands, useful to calculate the average electrophoretic mobility (AEM) of the HS and (ii) buffers with interactive agents (i.e. borate, urea, SDS, EDTA) that, separating several peaks or bands, permit a “finger-print” characterization of HSs. In the present paper we report some results about the characterization of peat humic acids (HA) fractions obtained by tangential ultra-filtration by CZE in order to improve the knowledge about electrophoretic behavior of HS and the application of CZE to the analysis of organic matter extracted from organic fertilizers.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
