In this chapter, a selected number of applications regarding the use of co-solvents and mobile phase additives to improve the quality of RP-HPLC applications are described. The beneficial effects deriving from the presence in the eluent of fluorinated ion-pairing reagents (carboxylic acids, amines, and alcohols), ionic liquids, deep eutectic solvents, chaotropic and kosmotropic agents, and surfactants are discussed with studies retrieved from the literature. All the sections included in the chapter provide evidence about the remarkable impact that some additives and co-solvents can have on both the thermodynamic and the kinetic features of the LC processes, in turn highlighting that the mobile phase components are deeply involved in the overall chromatographic process at multiple levels, even if present at low concentrations.
Protti M., Carotti A., Mercolini L., Sardella R. (2023). Chapter 12 - Co-solvents and mobile phase additives in HPLC. Amsterdam : Elsevier [10.1016/B978-0-323-99968-7.00025-4].
Chapter 12 - Co-solvents and mobile phase additives in HPLC
Protti M.Primo
;Mercolini L.
Ultimo
;
2023
Abstract
In this chapter, a selected number of applications regarding the use of co-solvents and mobile phase additives to improve the quality of RP-HPLC applications are described. The beneficial effects deriving from the presence in the eluent of fluorinated ion-pairing reagents (carboxylic acids, amines, and alcohols), ionic liquids, deep eutectic solvents, chaotropic and kosmotropic agents, and surfactants are discussed with studies retrieved from the literature. All the sections included in the chapter provide evidence about the remarkable impact that some additives and co-solvents can have on both the thermodynamic and the kinetic features of the LC processes, in turn highlighting that the mobile phase components are deeply involved in the overall chromatographic process at multiple levels, even if present at low concentrations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
