Accumulation of cyclin D1/cdk4/6 complexes in early to mid-G1 phase leads to the activation of the kinases that phosphorylate and inactivate the tumor suppressor Rb, which is required for cell cycle progression through the G1 to S phase. Previous studies suggested that focal adhesion kinase (FAK) may play a role in the regulation of cell cycle progression, indicating that FAK could increase cyclin D1 expression and/or decrease the expression of the cdk inhibitor p21. FAK is a nonreceptor protein-tyrosine kinase that controls proliferation, cell spreading, motility, and survival Recently, real time PCR analysis of matched samples of normal colon mucosa, colorectal carcinoma, and liver metastases demonstrated increased FAK mRNA and protein levels in tumor and metastatic tissues versus normal tissues. In this work we analyze the effects induced by 9-hydroxystearic acid (9HSA) in HCT116 on cell cycle and cytoskeleton organization. The hydroxy acid is a new identified histone deacetylase 1 inhibitor, and belongs to the class of lipid peroxidation by-products that greatly diminish in tumours
EFFECTS OF 9-HYDROXYSTEARIC ACID ON CELL PROLIFERATION AND CYTOSKELETON ORGANIZATION IN HCT116 COLON CARCINOMA CELLS.
PAGNOTTA, ELEONORA;CALONGHI, NATALIA;BOGA, CARLA;FARRUGGIA, GIOVANNA;MASOTTI, LANFRANCO
2005
Abstract
Accumulation of cyclin D1/cdk4/6 complexes in early to mid-G1 phase leads to the activation of the kinases that phosphorylate and inactivate the tumor suppressor Rb, which is required for cell cycle progression through the G1 to S phase. Previous studies suggested that focal adhesion kinase (FAK) may play a role in the regulation of cell cycle progression, indicating that FAK could increase cyclin D1 expression and/or decrease the expression of the cdk inhibitor p21. FAK is a nonreceptor protein-tyrosine kinase that controls proliferation, cell spreading, motility, and survival Recently, real time PCR analysis of matched samples of normal colon mucosa, colorectal carcinoma, and liver metastases demonstrated increased FAK mRNA and protein levels in tumor and metastatic tissues versus normal tissues. In this work we analyze the effects induced by 9-hydroxystearic acid (9HSA) in HCT116 on cell cycle and cytoskeleton organization. The hydroxy acid is a new identified histone deacetylase 1 inhibitor, and belongs to the class of lipid peroxidation by-products that greatly diminish in tumoursI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
