Avian pneumovirus causes an upper respiratory tract infection in turkeys leading to turkey rhinotracheitis (TRT) and in some other avian species, including chickens where it is also involved, in the etiology of multifactorial diseases such as swollen head syndrome. Since the 1980s a number of live attenuated subtype A and B APV vaccines have been developed in Europe and these have generally afforded good protection. All have been produced by repeated passages of field virus in a laboratory culture system to achieve various degrees of attenuation. They are widely used in commercial growing turkeys and to prime future layers and breeders. When tested under experimental conditions, these empirically derived vaccines were shown to be fully protective whilst not causing detectable disease themselves. However, they have not performed as well when used in the field and unstable attenuation has been considered to be a possible factor. Since pneumoviruses are single-stranded RNA viruses their relatively high mutation rates have been thought to be the underlying reason for instances of reversion to virulence observed in experimental conditions (1). This paper describes the first evidence of reversion of an APV vaccine in the field. First, systematic evidence of extended vaccine virus persistence in the field arose from a longitudinal study performed in the UK in 1995. Secondly, from an outbreak of TRT in an Italian turkey flock previously vaccinated at day old, we isolated an APV which proved to be a vaccine derivative. Finally, in order to determine whether vaccine virus or a derivative of increased virulence had been isolated, the virus was applied to 1 day old poults in secure isolation conditions. The vaccine derivative virus was shown to be able to cause clinical disease.
Catelli E., Cecchinato M., Savage C.E., De Matteo P., Franciosi C., Jones R.C., et al. (2005). FIELD AND EXPERIMENTAL EVIDENCES OF AVIAN PNEUMOVIRUS VACCINE REVERSION TO VIRULENCE. s.l : Omnipress.
FIELD AND EXPERIMENTAL EVIDENCES OF AVIAN PNEUMOVIRUS VACCINE REVERSION TO VIRULENCE
CATELLI, ELENA;CECCHINATO, MATTIA;DE MATTEO, PATRIZIA;FRANCIOSI, CARLO;
2005
Abstract
Avian pneumovirus causes an upper respiratory tract infection in turkeys leading to turkey rhinotracheitis (TRT) and in some other avian species, including chickens where it is also involved, in the etiology of multifactorial diseases such as swollen head syndrome. Since the 1980s a number of live attenuated subtype A and B APV vaccines have been developed in Europe and these have generally afforded good protection. All have been produced by repeated passages of field virus in a laboratory culture system to achieve various degrees of attenuation. They are widely used in commercial growing turkeys and to prime future layers and breeders. When tested under experimental conditions, these empirically derived vaccines were shown to be fully protective whilst not causing detectable disease themselves. However, they have not performed as well when used in the field and unstable attenuation has been considered to be a possible factor. Since pneumoviruses are single-stranded RNA viruses their relatively high mutation rates have been thought to be the underlying reason for instances of reversion to virulence observed in experimental conditions (1). This paper describes the first evidence of reversion of an APV vaccine in the field. First, systematic evidence of extended vaccine virus persistence in the field arose from a longitudinal study performed in the UK in 1995. Secondly, from an outbreak of TRT in an Italian turkey flock previously vaccinated at day old, we isolated an APV which proved to be a vaccine derivative. Finally, in order to determine whether vaccine virus or a derivative of increased virulence had been isolated, the virus was applied to 1 day old poults in secure isolation conditions. The vaccine derivative virus was shown to be able to cause clinical disease.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.