This analysis method comprises: an inflow step for causing a solution to flow into a flow cell, said solution including a test substance, a magnetic support body having, formed on the surface thereof, a complex including an antibody that is for recognizing the test substance and is labeled with a luminescent agent, and a reaction aid for aiding in the reaction of the luminescent agent; a capturing step for capturing the magnetic support body on a working electrode using a magnetic field from a magnetic field generation device; a light emission step for causing the luminescent agent to emit light by applying a voltage to the working electrode; and a measurement step for measuring the amount of light emitted by the luminescent agent. The light emission step includes light emission caused by action on the luminescent agent of first neutral radicals produced from cationic radicals from the reaction aid and light emission caused by the action on the luminescent agent of second neutral radicals produced directly from the reaction aid. As a result, it is possible to increase ECL light emission efficiency and enhance detection sensitivity.

BIOMOLECULE ANALYSIS METHOD AND BIOMOLECULE ANALYSIS DEVICE / Toshiro Saito, Kyoko Imai, Francesco Paolucci, Massimo Marcaccio, Giovanni Valenti, Alessandra Zanut, Michaela Windfuhr, Hans-Peter Josel. - (2019).

BIOMOLECULE ANALYSIS METHOD AND BIOMOLECULE ANALYSIS DEVICE

Francesco Paolucci;Massimo Marcaccio;Giovanni Valenti;
2019

Abstract

This analysis method comprises: an inflow step for causing a solution to flow into a flow cell, said solution including a test substance, a magnetic support body having, formed on the surface thereof, a complex including an antibody that is for recognizing the test substance and is labeled with a luminescent agent, and a reaction aid for aiding in the reaction of the luminescent agent; a capturing step for capturing the magnetic support body on a working electrode using a magnetic field from a magnetic field generation device; a light emission step for causing the luminescent agent to emit light by applying a voltage to the working electrode; and a measurement step for measuring the amount of light emitted by the luminescent agent. The light emission step includes light emission caused by action on the luminescent agent of first neutral radicals produced from cationic radicals from the reaction aid and light emission caused by the action on the luminescent agent of second neutral radicals produced directly from the reaction aid. As a result, it is possible to increase ECL light emission efficiency and enhance detection sensitivity.
2019
PCT/JP2019/030018
BIOMOLECULE ANALYSIS METHOD AND BIOMOLECULE ANALYSIS DEVICE / Toshiro Saito, Kyoko Imai, Francesco Paolucci, Massimo Marcaccio, Giovanni Valenti, Alessandra Zanut, Michaela Windfuhr, Hans-Peter Josel. - (2019).
Toshiro Saito, Kyoko Imai, Francesco Paolucci, Massimo Marcaccio, Giovanni Valenti, Alessandra Zanut, Michaela Windfuhr, Hans-Peter Josel
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/915232
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact