This analysis method comprises: an inflow step for causing a solution to flow into a flow cell, said solution including a test substance, a magnetic support body having, formed on the surface thereof, a complex including an antibody that is for recognizing the test substance and is labeled with a luminescent agent, and a reaction aid for aiding in the reaction of the luminescent agent; a capturing step for capturing the magnetic support body on a working electrode using a magnetic field from a magnetic field generation device; a light emission step for causing the luminescent agent to emit light by applying a voltage to the working electrode; and a measurement step for measuring the amount of light emitted by the luminescent agent. The light emission step includes light emission caused by action on the luminescent agent of first neutral radicals produced from cationic radicals from the reaction aid and light emission caused by the action on the luminescent agent of second neutral radicals produced directly from the reaction aid. As a result, it is possible to increase ECL light emission efficiency and enhance detection sensitivity.
BIOMOLECULE ANALYSIS METHOD AND BIOMOLECULE ANALYSIS DEVICE
Francesco Paolucci;Massimo Marcaccio;Giovanni Valenti;
2019
Abstract
This analysis method comprises: an inflow step for causing a solution to flow into a flow cell, said solution including a test substance, a magnetic support body having, formed on the surface thereof, a complex including an antibody that is for recognizing the test substance and is labeled with a luminescent agent, and a reaction aid for aiding in the reaction of the luminescent agent; a capturing step for capturing the magnetic support body on a working electrode using a magnetic field from a magnetic field generation device; a light emission step for causing the luminescent agent to emit light by applying a voltage to the working electrode; and a measurement step for measuring the amount of light emitted by the luminescent agent. The light emission step includes light emission caused by action on the luminescent agent of first neutral radicals produced from cationic radicals from the reaction aid and light emission caused by the action on the luminescent agent of second neutral radicals produced directly from the reaction aid. As a result, it is possible to increase ECL light emission efficiency and enhance detection sensitivity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
