In the last years, the Scanning Force Microscope imposed as a new and useful tool for the analysis of the structure of nucleic acids. The applications of this class of techniques goes from high resolution imaging of the double-helix, to the study of DNA-protein complexes, to the analysis of the dynamics of fully hydrated nucleic acids. In this chapter, we present an overview of the techniques and protocols available for the study of nucleic acids with the scanning force microscope. We devote particular attention to the study of the dynamics, the curvature and the flexibility of double-stranded DNA.
Scanning Force Microscopy studies on the structure and dynamics of single DNA molecules
Giampaolo ZuccheriPrimo
Writing – Original Draft Preparation
;
2002
Abstract
In the last years, the Scanning Force Microscope imposed as a new and useful tool for the analysis of the structure of nucleic acids. The applications of this class of techniques goes from high resolution imaging of the double-helix, to the study of DNA-protein complexes, to the analysis of the dynamics of fully hydrated nucleic acids. In this chapter, we present an overview of the techniques and protocols available for the study of nucleic acids with the scanning force microscope. We devote particular attention to the study of the dynamics, the curvature and the flexibility of double-stranded DNA.File in questo prodotto:
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