MOR expression levels at a specific cell type or tissue significantly contribute to its role in pain transmission and in other responses involving opioid receptors. Therefore, molecular processes regulating MOR levels have gained more and more interest. Recently, posttranscriptional regulation mechanisms have been shown to play a relevant role in influencing MOR expression levels, with polymorphisms and mutations within OPRM1 3′-UTR region impacting the differential opioid-mediated response observed within individuals. Here we report a Renilla luciferase reporter assay format suitable for dissecting the contribution of different and distinct OPRM1 3′-UTR elements to MOR expression levels in a model of glial cells, both under basal conditions and following specific treatments.
Vincelli G., Bedini A. (2021). Renilla luciferase reporter assay to study 3′-utr-driven posttranscriptional regulation of oprm1. New York : Humana Press Inc. [10.1007/978-1-0716-0884-5_2].
Renilla luciferase reporter assay to study 3′-utr-driven posttranscriptional regulation of oprm1
Vincelli G.;Bedini A.
2021
Abstract
MOR expression levels at a specific cell type or tissue significantly contribute to its role in pain transmission and in other responses involving opioid receptors. Therefore, molecular processes regulating MOR levels have gained more and more interest. Recently, posttranscriptional regulation mechanisms have been shown to play a relevant role in influencing MOR expression levels, with polymorphisms and mutations within OPRM1 3′-UTR region impacting the differential opioid-mediated response observed within individuals. Here we report a Renilla luciferase reporter assay format suitable for dissecting the contribution of different and distinct OPRM1 3′-UTR elements to MOR expression levels in a model of glial cells, both under basal conditions and following specific treatments.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
