Bioluminescence resonance energy transfer (BRET) is a very sensitive technique employed to study protein–protein interactions, including G-protein-coupled receptor (GPCR) hetero- and homo-dimerization. Recently, BRET has also been used to investigate the interaction between GPCRs (e.g.: α2 adrenergic receptor, muscarinic M2 receptor, dopaminergic D2 receptor) and nonvisual arrestins. Within the last decade an increasing interest arose toward opioid agonists with limited activation of arrestin-dependent signaling pathways, as they are believed to be effective analgesics with reduced adverse effects. Here a BRET protocol is described to investigate interactions between the kappa opioid receptor (KOR) and nonvisual arrestins (arrestin-2 and arrestin-3) in HEK-293 cells, both under basal conditions and after exposure to KOR ligands.
Bedini A. (2021). Bioluminescence resonance energy transfer (bret) to detect the interactions between kappa opioid receptor and nonvisual arrestins. New York : Humana Press Inc. [10.1007/978-1-0716-0884-5_5].
Bioluminescence resonance energy transfer (bret) to detect the interactions between kappa opioid receptor and nonvisual arrestins
Bedini A.
2021
Abstract
Bioluminescence resonance energy transfer (BRET) is a very sensitive technique employed to study protein–protein interactions, including G-protein-coupled receptor (GPCR) hetero- and homo-dimerization. Recently, BRET has also been used to investigate the interaction between GPCRs (e.g.: α2 adrenergic receptor, muscarinic M2 receptor, dopaminergic D2 receptor) and nonvisual arrestins. Within the last decade an increasing interest arose toward opioid agonists with limited activation of arrestin-dependent signaling pathways, as they are believed to be effective analgesics with reduced adverse effects. Here a BRET protocol is described to investigate interactions between the kappa opioid receptor (KOR) and nonvisual arrestins (arrestin-2 and arrestin-3) in HEK-293 cells, both under basal conditions and after exposure to KOR ligands.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.