Molecular analyses were carried out using two markers for Flavescence doreée (FD) phytoplasmas covering 16S ribosomal plus spacer region and translocase genes, in order to study FD strains present in Italy, Serbia, and France. The results confirm that Serbian FD strains belong to 16SrV-C subgroup. Both 16SrV-C and -D subgroups were still detected in Italy, and strains from both subgroups were studied in Veneto, while in Emilia and Tuscany 16SrV-D and 16SrV-C strains were respectively studied. Three of the four FD strains from France belong to 16SrV-D subgroup, while one was differentiable on 16S ribosomal gene from all others studied. By RFLP analyses on traslocase gene it was possible to differentiate subtypes in both ribosomal subgroups confirming ability of FD phytoplasmas to differentiate genotypes.
Molecular identification and geographic distribution of Flavescence dorée phytoplasma strains.
BERTACCINI, ASSUNTA;PALTRINIERI, SAMANTA;DUDUK, BOJAN
2009
Abstract
Molecular analyses were carried out using two markers for Flavescence doreée (FD) phytoplasmas covering 16S ribosomal plus spacer region and translocase genes, in order to study FD strains present in Italy, Serbia, and France. The results confirm that Serbian FD strains belong to 16SrV-C subgroup. Both 16SrV-C and -D subgroups were still detected in Italy, and strains from both subgroups were studied in Veneto, while in Emilia and Tuscany 16SrV-D and 16SrV-C strains were respectively studied. Three of the four FD strains from France belong to 16SrV-D subgroup, while one was differentiable on 16S ribosomal gene from all others studied. By RFLP analyses on traslocase gene it was possible to differentiate subtypes in both ribosomal subgroups confirming ability of FD phytoplasmas to differentiate genotypes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
