Soil-borne cereal mosaic virus (SBCMV), a Furovirus transmitted by Polymyxa graminis Led., is the causal agent of an important disease of wheat, widespread in Europe, where it causes losses in grain yield up to 70% with a great detrimental effects also on grain quality. Growing resistant cultivars represents the only effective and sustainable means of control. Although valuable sources of resistance have been identified, little information is available on the genetic location of resistance determinants. This work aims to determine the genetic basis of resistance to SBCMV in the durum wheat cultivar Neodur by means of a bulk segregant analysis (BSA) approach. A population of 200 F8 recombinant inbred lines (RILs) obtained from the cross between durum wheat cultivars Neodur (highly resistant) and Cirillo (highly susceptible) was evaluated for SBCMV infection severity in a field with natural inoculum sources of SBCMV, located near Bologna (Italy), during the 2007-08 season. The infection severity was evaluated by visual scoring of symptoms and DAS-ELISA assay. Ten susceptible and ten resistant lines were selected and utilised to produce susceptible and resistant DNA bulks which were analysed with more than 200 microsatellite markers polymorphic between the parents. Results of the polymorphism analysis on bulks indicate the presence of a major gene on the short arm of chromosome 2B controlling the resistant trait; detailed results and microsatellites markers suitable for transfer of the resistance will be presented.

Genetic mapping of a gene for resistance to SBCMV in the durum wheat cultivar Neodur.

RUBIES AUTONELL, CONCEPCION;RATTI, CLAUDIO
2009

Abstract

Soil-borne cereal mosaic virus (SBCMV), a Furovirus transmitted by Polymyxa graminis Led., is the causal agent of an important disease of wheat, widespread in Europe, where it causes losses in grain yield up to 70% with a great detrimental effects also on grain quality. Growing resistant cultivars represents the only effective and sustainable means of control. Although valuable sources of resistance have been identified, little information is available on the genetic location of resistance determinants. This work aims to determine the genetic basis of resistance to SBCMV in the durum wheat cultivar Neodur by means of a bulk segregant analysis (BSA) approach. A population of 200 F8 recombinant inbred lines (RILs) obtained from the cross between durum wheat cultivars Neodur (highly resistant) and Cirillo (highly susceptible) was evaluated for SBCMV infection severity in a field with natural inoculum sources of SBCMV, located near Bologna (Italy), during the 2007-08 season. The infection severity was evaluated by visual scoring of symptoms and DAS-ELISA assay. Ten susceptible and ten resistant lines were selected and utilised to produce susceptible and resistant DNA bulks which were analysed with more than 200 microsatellite markers polymorphic between the parents. Results of the polymorphism analysis on bulks indicate the presence of a major gene on the short arm of chromosome 2B controlling the resistant trait; detailed results and microsatellites markers suitable for transfer of the resistance will be presented.
Proceedings of the 53rd Italian Society of Agricultural Genetics Annual Congress (SIGA)
Poster Abst...
Poster Abst...
Russo M. A.; Marone D.; De Vita P.; Vallega V.; Rubies Autonell C; Ratti C.;
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/83894
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