Objectives: Calcium silicate cements (portland-derived cements and MTAs) are hydraulic cements capable of calcium release during their hydration and setting reaction when soaked in water and body fluids. They were developed as root-end filling materials but their clinical applications was extended to various situations, like perforation, pulp capping and apexification. The aim of this study was to evaluate the bioactivity (expressed by surface morphological modifications and chemical transformations) of two calcium-silicate cements soaked in Simulated Body Fluid (SBF) phosphate-containing. Materials and Methods: ProRoot MTA (Dentsply, USA) and experimental calcium-silicate cement (Retro TC, Italy) were used. The surface morphology and chemical properties of freshly prepared cements and of 14-days aged cements were investigated with micro-Raman, FTIR-ATR and ESEM/EDX. The ageing was performed by soaking the samples in 5ml of phosphate containing solution (SBF) for different times till 14 days. Samples in sealed holder were stored at 37°C. Results: ESEM/EDX analysis of freshly prepared cements showed the formation of needle-like crystals network on the surface. After 24 hours spherical precipitates (spherulites) deposited on the surface. The aggregation of these globular micro-crystals created bigger formations forming a surface coating. After 14 days of soaking the samples surface appeared irregular and porous. Raman analysis of fresh samples emphasized the typical bands of portland cement components, that corresponded to alite, belite and ettringite. After a 24 hours of soaking, Raman and FTIR analysis registered the same bands with a better-defined profile and the appearance of phosphate bands. Aged samples displayed calcium-phosphate bands (carbonate-apatite). Conclusions: this study demonstrated the bioactivity of calcium-silicate cements consisting in the ability to produce bone-like apatite when surrounded by a simulated body fluid. Calcium ions released from the cement react with phosphate ions from the solution inducing the formation of calcium phosphate deposits which stratify on the cement surface (biocoating).

Apatite spherulites and biocoating formation on calcium-silicate cements.

GANDOLFI, MARIA GIOVANNA;SIBONI, FRANCESCO;MODENA, ENRICO;TINTI, ANNA;TADDEI, PAOLA;ROSSI, PIERMARIA LUIGI;PRATI, CARLO
2009

Abstract

Objectives: Calcium silicate cements (portland-derived cements and MTAs) are hydraulic cements capable of calcium release during their hydration and setting reaction when soaked in water and body fluids. They were developed as root-end filling materials but their clinical applications was extended to various situations, like perforation, pulp capping and apexification. The aim of this study was to evaluate the bioactivity (expressed by surface morphological modifications and chemical transformations) of two calcium-silicate cements soaked in Simulated Body Fluid (SBF) phosphate-containing. Materials and Methods: ProRoot MTA (Dentsply, USA) and experimental calcium-silicate cement (Retro TC, Italy) were used. The surface morphology and chemical properties of freshly prepared cements and of 14-days aged cements were investigated with micro-Raman, FTIR-ATR and ESEM/EDX. The ageing was performed by soaking the samples in 5ml of phosphate containing solution (SBF) for different times till 14 days. Samples in sealed holder were stored at 37°C. Results: ESEM/EDX analysis of freshly prepared cements showed the formation of needle-like crystals network on the surface. After 24 hours spherical precipitates (spherulites) deposited on the surface. The aggregation of these globular micro-crystals created bigger formations forming a surface coating. After 14 days of soaking the samples surface appeared irregular and porous. Raman analysis of fresh samples emphasized the typical bands of portland cement components, that corresponded to alite, belite and ettringite. After a 24 hours of soaking, Raman and FTIR analysis registered the same bands with a better-defined profile and the appearance of phosphate bands. Aged samples displayed calcium-phosphate bands (carbonate-apatite). Conclusions: this study demonstrated the bioactivity of calcium-silicate cements consisting in the ability to produce bone-like apatite when surrounded by a simulated body fluid. Calcium ions released from the cement react with phosphate ions from the solution inducing the formation of calcium phosphate deposits which stratify on the cement surface (biocoating).
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M.G. Gandolfi; A. Colin; F. Siboni; E. Modena; A. Tinti; P. Taddei; P.L. Rossi; C. Prati
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/83453
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