CYP3A enzyme is a toxicologically relevant end-point; interactions between xenobiotics (e.g. pharmaceuticals and xenoestrogens) and CYP3A may compromise both the clearance of xenobiotics and endogenous molecules (e.g. steroid hormones), as they share common routes of metabolism through CYP3A enzymes. The purpose of this work was to better characterize CYP3A-like enzyme in microsomal fractions isolated from the digestive gland of mussels Mytilus galloprovincialis by using 7benzyloxy-4-trifluoromethylcoumarin as a substrate. The higher Km value in mussels collected from Barcelona harbor with respect to that collected from Ebro delta indicated lower affinity of the enzyme in polluted specimens. 17 alfa-ethynylestradiol (EE2)acted as mixed inhibitor of CYP3A activity. The study contributes to the better knowledge of CYP3A-like activity in mussels and shows potential interference between EE2 and possibly other pollutants, and CYP3A function.

M.Thibaut, S. Nesci, A. Pagliarani, C. Porte (2009). Characterization of a CYP3A-like activity in the digestive gland of Mytilus galloprovincialis-inhibition by 17alfa ethynylestradiol. BORDEAUX : s.n.

Characterization of a CYP3A-like activity in the digestive gland of Mytilus galloprovincialis-inhibition by 17alfa ethynylestradiol

NESCI, SALVATORE;PAGLIARANI, ALESSANDRA;
2009

Abstract

CYP3A enzyme is a toxicologically relevant end-point; interactions between xenobiotics (e.g. pharmaceuticals and xenoestrogens) and CYP3A may compromise both the clearance of xenobiotics and endogenous molecules (e.g. steroid hormones), as they share common routes of metabolism through CYP3A enzymes. The purpose of this work was to better characterize CYP3A-like enzyme in microsomal fractions isolated from the digestive gland of mussels Mytilus galloprovincialis by using 7benzyloxy-4-trifluoromethylcoumarin as a substrate. The higher Km value in mussels collected from Barcelona harbor with respect to that collected from Ebro delta indicated lower affinity of the enzyme in polluted specimens. 17 alfa-ethynylestradiol (EE2)acted as mixed inhibitor of CYP3A activity. The study contributes to the better knowledge of CYP3A-like activity in mussels and shows potential interference between EE2 and possibly other pollutants, and CYP3A function.
2009
Abstract Book
456
456
M.Thibaut, S. Nesci, A. Pagliarani, C. Porte (2009). Characterization of a CYP3A-like activity in the digestive gland of Mytilus galloprovincialis-inhibition by 17alfa ethynylestradiol. BORDEAUX : s.n.
M.Thibaut; S. Nesci; A. Pagliarani; C. Porte
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/82302
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact