CYP3A enzyme is a toxicologically relevant end-point; interactions between xenobiotics (e.g. pharmaceuticals and xenoestrogens) and CYP3A may compromise both the clearance of xenobiotics and endogenous molecules (e.g. steroid hormones), as they share common routes of metabolism through CYP3A enzymes. The purpose of this work was to better characterize CYP3A-like enzyme in microsomal fractions isolated from the digestive gland of mussels Mytilus galloprovincialis by using 7benzyloxy-4-trifluoromethylcoumarin as a substrate. The higher Km value in mussels collected from Barcelona harbor with respect to that collected from Ebro delta indicated lower affinity of the enzyme in polluted specimens. 17 alfa-ethynylestradiol (EE2)acted as mixed inhibitor of CYP3A activity. The study contributes to the better knowledge of CYP3A-like activity in mussels and shows potential interference between EE2 and possibly other pollutants, and CYP3A function.
Characterization of a CYP3A-like activity in the digestive gland of Mytilus galloprovincialis-inhibition by 17alfa ethynylestradiol / M.Thibaut; S. Nesci; A. Pagliarani; C. Porte. - STAMPA. - (2009), pp. 456-456. (Intervento presentato al convegno 15th International Symposium on Pollutant Responses in Marine Organisms (PRIMO) tenutosi a Bordeaux, France nel 17-20 Maggio 2009).
Characterization of a CYP3A-like activity in the digestive gland of Mytilus galloprovincialis-inhibition by 17alfa ethynylestradiol
NESCI, SALVATORE;PAGLIARANI, ALESSANDRA;
2009
Abstract
CYP3A enzyme is a toxicologically relevant end-point; interactions between xenobiotics (e.g. pharmaceuticals and xenoestrogens) and CYP3A may compromise both the clearance of xenobiotics and endogenous molecules (e.g. steroid hormones), as they share common routes of metabolism through CYP3A enzymes. The purpose of this work was to better characterize CYP3A-like enzyme in microsomal fractions isolated from the digestive gland of mussels Mytilus galloprovincialis by using 7benzyloxy-4-trifluoromethylcoumarin as a substrate. The higher Km value in mussels collected from Barcelona harbor with respect to that collected from Ebro delta indicated lower affinity of the enzyme in polluted specimens. 17 alfa-ethynylestradiol (EE2)acted as mixed inhibitor of CYP3A activity. The study contributes to the better knowledge of CYP3A-like activity in mussels and shows potential interference between EE2 and possibly other pollutants, and CYP3A function.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
