Endothelin-1 (ET-1) has gained increasing attention due to its involvement in angiogenesis; for example, ET-1 plays a role in corpus luteum (CL) lifespan by regulating both its formation and regression. It is well-known that fasting influences ovarian angiogenesis by modulating the expression of angiogenic factors, such as VEGF, in both follicle and CL. The present study was aimed at investigating whether fasting influences ET-1 system in porcine CL. Eight prepubertal gilts were treated with 1250 IU eCG (day 0) followed 60h later by 750 IU hCG in order to induce follicle growth and ovulation. At day 5 the animals were randomly assigned to either a control (n=4, normal alimentation) or a fasted (72 h of fasting; n=4) group. At day 8 (end of fasting) ovaries were collected and CLs were counted and processed singularly for mRNA extraction; all the determinations were carried out on 10 CLs/gilt. Swine primers were designed for ET-1, ET-1 receptor type A (ET-A) and ET-1 Converting Enzyme (ECE-1) and quantitative Real-time RT-PCR was performed. The housekeeping beta-actin was used to normalise the amount of RNA. The ΔΔCT method was used to determine the relative amount of the target genes. ET-1 mRNA levels in CLs from fasted animals were significantly lower (p<0.005) than those from control gilts, with a relative decrease of 34%; similar results were observed for ECE-1 with a 20% reduction (p<0.05). No significant differences were observed for ET-A mRNA expression between the two groups. A positive correlation was observed between ET-1 and ET-A mRNA expression (r=0.795, p< 0.001), ET-1 and ECE-1 (r=0.664, p< 0.001) and between ET-A and ECE-1 (r=0.694, p< 0.001). Basing on these data, fasting seems to be effective in inhibiting the endothelin system in luteal tissue.
INFLUENCE OF FASTING ON ET-1 SYSTEM IN SWINE CORPORA LUTEA
FORNI, MONICA;ZANNONI, AUGUSTA;DE ANDREA RIBEIRO, LUCIANA;GOVONI, NADIA;SPINACI, MARCELLA;BACCI, MARIA LAURA;GALEATI, GIOVANNA;TAMANINI, CARLO
2004
Abstract
Endothelin-1 (ET-1) has gained increasing attention due to its involvement in angiogenesis; for example, ET-1 plays a role in corpus luteum (CL) lifespan by regulating both its formation and regression. It is well-known that fasting influences ovarian angiogenesis by modulating the expression of angiogenic factors, such as VEGF, in both follicle and CL. The present study was aimed at investigating whether fasting influences ET-1 system in porcine CL. Eight prepubertal gilts were treated with 1250 IU eCG (day 0) followed 60h later by 750 IU hCG in order to induce follicle growth and ovulation. At day 5 the animals were randomly assigned to either a control (n=4, normal alimentation) or a fasted (72 h of fasting; n=4) group. At day 8 (end of fasting) ovaries were collected and CLs were counted and processed singularly for mRNA extraction; all the determinations were carried out on 10 CLs/gilt. Swine primers were designed for ET-1, ET-1 receptor type A (ET-A) and ET-1 Converting Enzyme (ECE-1) and quantitative Real-time RT-PCR was performed. The housekeeping beta-actin was used to normalise the amount of RNA. The ΔΔCT method was used to determine the relative amount of the target genes. ET-1 mRNA levels in CLs from fasted animals were significantly lower (p<0.005) than those from control gilts, with a relative decrease of 34%; similar results were observed for ECE-1 with a 20% reduction (p<0.05). No significant differences were observed for ET-A mRNA expression between the two groups. A positive correlation was observed between ET-1 and ET-A mRNA expression (r=0.795, p< 0.001), ET-1 and ECE-1 (r=0.664, p< 0.001) and between ET-A and ECE-1 (r=0.694, p< 0.001). Basing on these data, fasting seems to be effective in inhibiting the endothelin system in luteal tissue.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
