The meningococcus genome is characterized by high plasticity promoted by spontaneous mutational mechanisms: local or global changes due to repeated sequences, phase variation, horizontal gene transfer and others. The CREN (contact regulatory element of Neisseria) is a 50-150bps repeated sequence localized in the genome of pathogenic Neisseria species and often located directed upstream of the ATG start codon of genes. This DNA repeat sequence was identified as a cis-regulatory element that coordinates the upregulation of several genes in response to host cell contact, however the mechanism through which this occurs remains obscure. In serogroup B meningococcal strain MC58 12 highly conserved CREN regions were identified. In our work we focus on nmb2132, one of the 5 MenB vaccine antigens and CrgA (nmb1856), with CREN element in their 5’UTR. We demonstrate that there is a co-regulation mediated by Hfq and CREN at the post-transcriptional level for genes with CREN in their 5’UTR.
Pigozzi E., Fantappiè L., Ieva R., Spadafina T., Serruto D., Seib K., et al. (2009). The involvement of mobile chromosomal elements in post-transcriptional regulatory control via the Hfq RNA chaperone. ROMA : SIMGBM.
The involvement of mobile chromosomal elements in post-transcriptional regulatory control via the Hfq RNA chaperone
FANTAPPIE', LAURA;SCARLATO, VINCENZO;
2009
Abstract
The meningococcus genome is characterized by high plasticity promoted by spontaneous mutational mechanisms: local or global changes due to repeated sequences, phase variation, horizontal gene transfer and others. The CREN (contact regulatory element of Neisseria) is a 50-150bps repeated sequence localized in the genome of pathogenic Neisseria species and often located directed upstream of the ATG start codon of genes. This DNA repeat sequence was identified as a cis-regulatory element that coordinates the upregulation of several genes in response to host cell contact, however the mechanism through which this occurs remains obscure. In serogroup B meningococcal strain MC58 12 highly conserved CREN regions were identified. In our work we focus on nmb2132, one of the 5 MenB vaccine antigens and CrgA (nmb1856), with CREN element in their 5’UTR. We demonstrate that there is a co-regulation mediated by Hfq and CREN at the post-transcriptional level for genes with CREN in their 5’UTR.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
