Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein–protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the discovery of new functions and new way of signaling and trafficking; therefore, opioid receptor oligomers may be considered as novel drug targets. Fusing receptors of interest with Renilla luciferase and with a fluorescent protein (such as EYFP) it is possible to study opioid receptor dimerization using BRET.
Baiula M. (2021). Monitoring opioid receptor interaction in living cells by bioluminescence resonance energy transfer (bret). New York : Humana Press Inc. [10.1007/978-1-0716-0884-5_4].
Monitoring opioid receptor interaction in living cells by bioluminescence resonance energy transfer (bret)
Baiula M.Primo
2021
Abstract
Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein–protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the discovery of new functions and new way of signaling and trafficking; therefore, opioid receptor oligomers may be considered as novel drug targets. Fusing receptors of interest with Renilla luciferase and with a fluorescent protein (such as EYFP) it is possible to study opioid receptor dimerization using BRET.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
